WANG Nengqiang, WU Jianping, ZOU Xiaoming, et al. Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis[J]. CIESC Journal, 2007, (1).
WANG Nengqiang, WU Jianping, ZOU Xiaoming, et al. Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis[J]. CIESC Journal, 2007, (1).DOI:
Two level factorials designs of Plackett-Burman were constructed to select the Pseudomonas W20 fermentation medium components of α-chloropropionic acid dehalogenases.Three important components were urea
glucose and KH2PO4.The optimized levels of these factors were determined by the response surface analysis. The results showed that the optimum concentrations of urea
glucose and KH2PO4 were 1.19 g·L-1
18.4 g·L-1 and 1.30 g·L-1 respectively.The enzyme activity increased by 23.77% under the optimized condition.