The gene dhaB encoding glycerol dehydrase from Citrobacter freundii gene dhaB and 1

3-propanediol (1

3-PD)oxidoreductase isoenzyme encoding gene (yqhD) were transferred into temperature control expression vector pHsh to construct a novel recombinant Escherichia coli JM109 (pHsh-dhaB-yqhD).Some nutrient ingredients affecting fermentation results of this recombinant strain were also studied.The results demonstrated that the optimized medium comprised(g·L-1) glycerol 60

yeast extract 5.0

KH2PO4 7.5 and vitamin B12 0.05.The 1

3-propanediol concentration

yield on glycerol and productivity in the 5 L fermenter could reach 43.26 g·L-1

72.2 % and 1.55 g·L-1·h-1 respectively.