The effects of two different phosphorus sources

corn steep liquor（CSL）and KH2PO4

on the growth of Candida glycerinogenes and glycerol production in batch fermentations were investigated. The glucose consumption in glycerol production

cell growth

maintenance

and byproducts formation during different fermentation phases was also analyzed kinetically.The results showed that the influence of CSL on cell growth and glucose consumption was so prominent that even minor variation of its quality or quantity caused much difference in experimental results. It was also found that the relationship between glycerol synthesis and cell growth was very close with CSL as phosphorus source

while it was rather loose with KH2PO4 as phosphorus source. With the increase in phosphorus concentration

glucose consumption in cell growth and maintenance was kept basically stable during the whole fermentation process. However

glucose consumption in glycerol production gradually decreased

and the byproducts which were mainly formed during the rapid growth phase increased. Further studies indicated that the yield of glycerol on glucose reached the peak of 53.44 % and 52.76 % with 14 g8226;L-1 CSL and 0.57 g8226;L-1 KH2PO4

respectively.Accordingly the yield of byproducts also decreased to the minimum value of 5.84 % and 4.25 %

respectively.