The effect of induction temperatures on porcine interferon-α(pIFN-α）production byPichia pastoris was investigated in a 10 L bioreactor with methanol concentration controlled at a specific level. The results indicated that a low temperature was obviously beneficial for pIFN-α expression

and the highest pIFN-α antiviral activity reached a level of 1.5×106 IU·ml-1 when induction was made at 20℃

that was 150-folds of the maximal value obtained with the traditional induction strategy at 30℃.On-line multi-variable measurement of the fermentative state variables and metabolic analysis showed that cell metabolic activity and specific methanol consumption rate (0.036 h-1）could be higher with induction at 20 Key words: 毕赤酵母；猪αFONT-FAMILY: Calibri

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mso-bidi-language: AR-SA">干扰素；诱导；代谢分析