Hydrophobic charge induction chromatography(HCIC)was used to separate immunoglobulin IgY from chicken plasma.The plasma was pretreated with caprylic acid to precipitate some impurities

and the supernatant was used directly as the feedstock for separation and purification of immunoglobulin IgY by HCIC.Two resins

commercial MEP HyperCel and homemade BestaroseDVSMMI

were used as HCIC medium and investigated.Operation conditions were optimized

including pH value of sample loading and elution

as well as sample loading volume.It was found that BestaroseDVSMMI with 2mercapto1methylimidazole as the ligand was better than MEP HyperCel.The results indicated that IgY could be separated with high purity(above 92% with HPLC analysis)and yield about 95%.The mechanism of HCIC was discussed based on comparison of ligand structure and surface potential.The results demonstrated that HCIC can be efficiently used for IgY purification from chicken plasma

which can be a new approach for the immunoglobulin separation from the plasma.