Microbes play an important role in bioremediation technologies

the aim of this article was to investigate functional strains for soil bio-remediation.In this study benzo［a］pyrene(BaP)

benzo［a］anthracene(BaA)

benzo［b］fluoranthene(BbF)

benzo［k］fluoranthene(BkF)

indeno［1

2

3-cd］pyrene(IPY) were used as carbon and energy sources and 50 strains were isolated by steps including enrichment culture and plate cultivation from the contaminated soil at a coking plant in Beijing.The result of 16S rRNA gene sequencing analysis revealed that these strains were clustered into 15 groups and belonged to genera Sphingomonas，Methylobacterium，Burkholderia，Rhodococcus，Bradyrhizobium，Phyllobacterium，Chryseobacterium，Microbacteriumm，of which Sphingomonas was the dominant species.The highest degradation rates of BaA，BaP，BbF，BkF，IPY were 39.64%，33.52%，38.57%，25.37% and 31.17% respectively when tested with strain 3-6-12.The experimental results will be useful for bioremediation of PAHs-contaminated soil.