Based on the principle of simultaneously denitrifying and short-cut sulfide removing，monosodium glutamate waste water sludge was used as inoculum，and the continuous stirred reactor was used to enrich the simultaneously denitrifying and short-cut sulfide removing bacteria.Traditional and modern molecular biology methods were adopted to determine the taxonomic status of the isolated bacteria which was named SNB.Response surface methodology was used to optimize the growth condition.The result showed that SNB had 99.0% homology with Thauera selenatis.Morphological and biochemical characteristics were also similar to Thauera selenatis.It belonged to short rod species.Thauera selenatis is not named in Chinese yet.Biochemical properties，stirred reactor reaction conditions and material balance showed that SNB was a kind of facultative anaerobic denitrifying and sulfide removing bacteria.The first and second items of pH were 0.0038 and 0.0095 respectively.The first and second items of peptone were 0.0868 and 0.447 respectively.The value of pH had a significant effect on the growth of SNB，peptone was the second.The best growth conditions were pH 8，the addition of peptone and sucrose was 1.88 g·L-1 nd 30.0 g·L-1 respectively.