Vitamin E succinate was synthesized via acylation of vitamin E with succinic anhydride using Candida sp.lipase as catalyst.The effects of enzyme sources，organic media and reaction temperature on the reaction yield were investigated.The results showed that Candida sp.lipase was the best lipase，the mixture of tert-butanol and DMSO (2∶3，vol) was the most suitable media for the reaction，and the appropriate reaction temperature was 30℃.Response surface methodology (RSM) and three-level-three-factor Box-Behnken design were used to evaluate the effects of other synthesis parameters，such as reaction time，substrates concentration and the molar ratio of vitamin E to succinic anhydride on the yield of vitamin E succinate.By solving the quadratic regression model equation using appropriate statistic methods，the optimum synthesis conditions were as follows：reaction time 71 h，vitamin E concentration 0.26 mmol·ml-1，vitamin E to succinic anhydride molar ratio 1∶5.The predicted value of the vitamin E succinate yield was 99.27%.The actual experimental value 98.71% was in good agreement of the predicted value.