Coal granular activated carbon with particle size distribution from 0.27 mm to 1.70 mm was used as adsorbent in a fixed-bed to separate deacetyl cephalosporin C（DCPC）from the waste drainage.The adsorbed DCPC on the carbon was eluted with 8%（vol）ethanol aqueous solution，and the obtained eluate contained DCPC in a yield of 60%～65% with a purity higher than 85% .After being further condensed through nanofilter membrane，the eluate was allowed to pass through anion-exchange resin Amberlite IRA67 on which DCPC was adsorbed.Desorption with 0.5 mol·L-1 sodium acetate buffer solution（pH6.3），DCPC was obtained as sodium salt solution in a yield of 90%～95% with 96% purity and the concentration was higher than 30 g·L-1.DCPC sodium salt was isolated by crystallization through adding acetone to the eluate.The adsorption capacity of the used coal granular activated carbon could be restored by saturation with alkali solution containing isopropanol and then neutralization with acidic solution.