The transesterification of Jatropha oil and methyl acetate to biodiesel catalyzed by a compound lipase was investigated. A 5-level-3-factor central composite rotatable design of the response surface method was used to evaluate the effects of compound enzyme amount

compound enzyme formulation and substrate composition on the yield of biodiesel. The optimum conditions were as follows: enzyme amount 0.27g

compound enzyme formulation 0.15 (mass) N234 and substrate composition of methyl acetate to Jatropha oil 10. 10. Under optimum conditions

the actual experimental yield was 74. 34%

which compared well with the predicted value of 72.55%. The model based on Ping Pong Bi Bi mechanism with substrate competitive inhibition was proposed to describe the reaction kinetics of the transesterification.