The study was based on the principle of shortcut denitrifying phosphorus removal.In order to enrich shortcut denitrifying phosphorus removing bacteria (SDPB)

anaerobic sludge was used in the anaerobic/anoxic SBR as inoculum.Anaerobic/anoxic phase was studied and nitrite was added as electronic acceptor.A SDPB strain was screened and isolated and the classification status was studied by adopting traditional and modern molecular identification methods.The biological utilization of the bacterial strain under different nutrition and environment conditions was investigated.The result showed that the bacterium was facultative anaerobe and bore simultaneous shortcut denitrifying/phosphorus removing function.It belonged to Acinetobacter sp.according to its morphological

physiology

biochemical

properties and the analysis of 16S rDNA gene sequence with 99.3% similarity.The optimum carbon resource was sodium acetate.The bacteria used not only nitrite but also nitrate as electronic acceptor.The best pH value was 7 and the best temperature was 25℃.The appropriate temperature was 20—35℃.The growth of micro-organism was inhibited when the temperature was 10℃.The activity of microorganism decreased when the temperature was above 35℃.The highest removal efficiency of phosphate and nitrate were 82.94% and 82.99%

respectively.