A new model of enzymatic synthesis of γ-D-Glu-L-Trp（SCV-07）from D-glutamine and L-tryptophan was developed.The Km and Kcat of transpeptidation and enzymatic hydrolysis of product (presented as K′m and K′cat) were determined as Km=5.11 mmol·L-1

Kcat =3.92 mmol·min-1

K′m=2.31 mmol·L-1 and K′cat=1.46 mmol·min-1.The sequential mechanism was used to describe the rate of transpeptidation，and the model parameters were optimized.The enzymatic synthesis of γ-D-glutamyl-L-tryptophan was investigated to verify the model.Good agreement between experimental data and calculations was obtained.The total relative errors of experimental data and calculations were less than 5%.