A simple UV spectroscopic method for determination of sugars was developed.Calibration models were established by the spectral measurement of a set of hydrolyzed standard sugar solutions by treating with concentrated sulfuric acid and a chemometrical software.The sugar contents in the sample can be predicted with the models by using the multi-wavelength spectral signals of its hydrolyzed solution.The present method does not need organic reagents and can provide the information of pentoses

hexoses

and total sugars in samples

and is suitable to be used for sugar analysis of the lignocellulosic biomass extraction process in bio-refinery research.