In the batch fermentation of pyruvate

temperature is one of the most important environmental factors that affect pyruvate productivity.The effect of temperature

varying from 26℃ to 34℃

on production of pyruvate in a 7 L stirred fermenter by T.glabrata CCTCC M202019 was investigated.A modified Logistic equation and Luedeking-Piret equation were used to estimate kinetics of cell growth and pyruvate production.The effect of temperature on the estimated kinetic parameters was further studied by regression analysis.Based on kinetic parameters analysis

a temperature-shifting strategy was proposed

in which

at 0—8 h

culturing was performed at 34℃ to obtain a high specific cell growth rate

and at 8—42 h

the temperature was decreased step by step from 34℃ to 27℃ to keep a high pyruvate production rate.After 42 h

the temperature was maintained at 27℃ to weaken inhibition of pyruvate and offer constant production capacity of pyruvate in the anaphase of fermentation.A high concentration (89.4 g8226;L-1)

yield (0.76 g8226;g-1) and productivity (1.32 g8226;L-18226;h-1) of pyruvate were achieved by applying this strategy

which were 25.7%

16.9% and 48.3% higher than batch fermentation at a constant temperature 30℃

respectively.