Chromatography is commonly used in separating expensive fine chemical products.Liquid chromatographic procedure is the essential tool in protein purification because of its mild condition

quick and effective separation.In recent years

chromatography has been gradually used in protein refolding from denatured state to biologically active conformation.In the liquid chromatography-based protein refolding procedure

a high yield could be achieved at high protein concentrations.Partial purification of protein could also be achieved simultaneously.The recent development of liquid chromatography-based protein refolding is reviewed in this paper.The principles

advantages

disadvantages and applications are discussed with respect to various chromatographic refolding processes

including size exclusion chromatography

ion exchange chromatography

hydrophobic interaction chromatography

affinity chromatography with immobilized refolding catalyst and artificial chaperone.