GAN Yiru, HUANG Yongdong, WU Lei. APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1[J]. CIESC Journal, 2004, (3): 497-500.
GAN Yiru, HUANG Yongdong, WU Lei. APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1[J]. CIESC Journal, 2004, (3): 497-500.DOI:
By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC)
thymosin α1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-PakTM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable