Expanded bed adsorption (EBA) has been widely used in the industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-TiO2 composite matrix was prepared with the method of water-in-oil suspension thermal regeneration. After activating by epichlorohydrin and coupling with diethylamine

the matrix was obtained to function as an anion exchanger. The experiments in expanded bed indicated that the adsorbent exhibited a stable expansion

approximate plug flow and the comparable column efficiency with the case in packed bed

providing a bovine serum albumin(BSA) adsorption capacity of 42.6 mg8226;ml-1 adsorbent at 10% breakthrough.