A new intravenous micro-bubble ultrasound contrast agent with three ester surfactants and other additives as its nano-shelled materials was prepared by sonication. Some paramemters in the process such as the selection of ultrasound power and time

the effect of the buffer pH value on the micro-bubble production

the adding style of the inner gas

the effect of different diluents on the micro-bubble flowing state

were studied. The preservation styles and in vitro contrast imaging of the micro-bubbles were investigated. Six dogs were used to verify the in vivo contrast effect of the prepared micro-bubble ultrasound contrast agent. The ultrasound contrast perfusion images of the dog’s kidney were gived.