The oxidation/adsorption properties of polyphenol oxidase（PPO）-celite coupled system in the separation of catechol from aqueous media containing anisole were studied.The enzyme showed exclusive substrate selectivity for catechol oxidation.Celite adsorbed the enzyme-generated products subsequently.The coexisting anisole presented no undesirable influence on the reaction.This phenomenon indicated that the coupled system enhanced the elimiation process of catechol.PPO can be easily separated from aqueous media.The half-life of the enzyme increased about 2.4 folds and the corresponding adsorption efficience could be as high as 98.76 %.