By electron paramagnetic resonance spectroscopy

electron microscopy and energy spectrum

the transferring of physiological state of cells was studied when oligosaccharide from Fusarium oxysprum was added in suspension cultures of Taxus chenensis var. mairei. Cells before elicitation were in a good state of growth including integration of cells

abundance of organelle

stability of active oxygen system

low redox potential of cells

flourishing of primary metabolism and low rate of taxol biosynthesis. After elicitation the activity of superoxide dismutase was improved sharply at first

and the activity of catalase and peroxidase were inhibited severely

then the oxidative burst was caused in cells and reached a maximum at 4h. The redox potential of cells was promoted rapidly

and ion channels in cell membrane were activated leading to H +

Ca 2+ influx and Cl - efflux which caused alkalization of medium. The observation by using optical microcopy and transmission electron microscopy showed that the high electron density body including Ca 2+ in vacuole changed regularly. Cell growth was inhibited

but productivity of taxol was increased 5.5 fold of that of control (76.1mg·L -1 ) and the system became the state of product synthesis.