Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO<sub>2</sub> nanoparticles

LIU Jie; SHEN Shaochuan; CHEN Ping; XU Linhong; YUN Junxian; YAO Kejian

doi:10.3969/j.issn.0438-1157.2014.10.026

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Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO₂ nanoparticles

更新时间：2025-12-10

- Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO₂ nanoparticles
- CIESC Journal Issue 10, Pages: 3938-3945(2014)
- 作者机构：
  
  1. 浙江工业大学化学工程学院绿色化学合成技术国家重点实验室培育基地,浙江,杭州,310032
  2. 中国地质大学（武汉）机械与电子信息学院,湖北,武汉,430074
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2014.10.026
  CLC： Q028
- Online First：05 October 2014，
  
  Published：05 October 2014
- 稿件说明：
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LIU Jie, SHEN Shaochuan, CHEN Ping, et al. Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO₂ nanoparticles[J]. CIESC Journal, 2014, (10): 3938-3945. DOI: 10.3969/j.issn.0438-1157.2014.10.026.
DOI：

LIU Jie, SHEN Shaochuan, CHEN Ping, et al. Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO₂ nanoparticles[J]. CIESC Journal, 2014, (10): 3938-3945. DOI: 10.3969/j.issn.0438-1157.2014.10.026. DOI：

摘要

用结晶致孔法制备了内嵌SiO2颗粒的聚甲基丙烯酸羟乙酯基复合晶胶，经甲基丙烯酸二甲氨乙酯接枝修饰后，得到了阴离子交换复合晶胶介质，用于从啤酒酵母转化液中色谱分离三磷酸胞苷。色谱分离过程以0.01 mol·L-1 HCl为平衡液，以0.02 mol·L-1 和0.1 mol·L-1 的NaCl溶液（用平衡液配制）分步洗脱。通过高效液相色谱定量分析，所得三磷酸胞苷的纯度达97.2%，回收率82.5%。与其他分离方法相比，本法过程简单、分离迅速高效，在核苷酸药物的分离领域具有很好的应用前景。

Abstract

An anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO2 nanoparticles was prepared by cryo-polymerization followed by graft polymerization with monomer N

N-dimethylaminoethyl methacrylate. This cryogel was used to isolate cytidine triphosphate from Saccharomyces cerevisiae transformation broth. Chromatographic separation was performed with 0.01 mol·L-1 HCl as running buffer together with 0.02 mol·L-1 and 0.1 mol·L-1 NaCl solutions (prepared in running buffer) as two-step elution liquids. Cytidine triphosphate purity was analyzed by high performance liquid chromatography. Cytidine triphosphate with high purity of 97.2% and recovery of 82.5% was obtained. Compared with other conventional methods

the present separation process was simple and effective

and could be used in separation of nucleotides from microbial fermentation or transformation feedstocks.

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Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO2 nanoparticles

DOI：10.3969/j.issn.0438-1157.2014.10.026

摘要

Abstract

关键词

Keywords

references

Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with SiO₂ nanoparticles