Construction of heat resistance devices for Escherichia coli and their application

SUN Xiangying; LIU Yueqin; SUN Huan; JIA Haiyang; DAI Dazhang; LI Chun

doi:10.3969/j.issn.0438-1157.2014.08.038

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Construction of heat resistance devices for Escherichia coli and their application

更新时间：2025-12-10

- Construction of heat resistance devices for Escherichia coli and their application
- CIESC Journal Issue 8, Pages: 3128-3135(2014)
- 作者机构：
  
  1. 石河子大学化学化工学院/兵团绿色化工过程重点实验室,新疆,石河子,832003
  2. 北京理工大学生命学院生物工程系,北京,100081
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2014.08.038
  CLC： 789
- Online First：05 August 2014，
  
  Published：05 August 2014
- 稿件说明：
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SUN Xiangying, LIU Yueqin, SUN Huan, et al. Construction of heat resistance devices for Escherichia coli and their application[J]. CIESC Journal, 2014, (8): 3128-3135.
DOI：

SUN Xiangying, LIU Yueqin, SUN Huan, et al. Construction of heat resistance devices for Escherichia coli and their application[J]. CIESC Journal, 2014, (8): 3128-3135. DOI： 10.3969/j.issn.0438-1157.2014.08.038.

摘要

以提高大肠杆菌耐热性为目的，基于腾冲嗜热菌（Thermoanaerobacter tengcongensis MB4）热激蛋白基因 T.te-HSP20 构建了诱导型耐热元器件T7-T.te-HSP20 和组成型耐热元器件gapA-T.te-HSP20，转入大肠杆菌（Escherichia coli）获得工程菌 E. coli-TH 和 E. coli-GH。工程菌E. coli-TH在30℃和IPTG诱导下，目标蛋白呈可溶性表达，经50℃热激30 min后，存活率提高了3.2倍。高温发酵表明gapA-T.te-HSP20扩宽了工程菌E. coli-GH的最适生长温度的范围（37～43℃），较大程度提高了大肠杆菌的耐热性。抗逆性分析还发现工程菌E. coli-GH具备了耐热与耐丁醇的双重功能，并有一定的抗乙酸和乙醇能力。为工业梯度升温发酵生产生物基产品的高效制造、节省成本提供了新思路。

Abstract

To improve the heat resistance of Escherichia coli

an inducible heat-resistance device T7-T.te-HSP20 and a constitutive heat-resistance device gapA-T.te-HSP20 based on T.te-HSP20 gene from Thermoanaerobacter tengcongensis MB4

and corresponding engineered strains E. coli-TH and E. coli-GH were constructed. The targeted protein was expressed in solubility after IPTG induction at 30℃ in E. coli-TH. Meanwhile

the survival rate of E. coli-TH was 3.2 times higher than the control at 50℃ for 30 min. The result of high-temperature fermentation showed that the optimum temperature range of E. coli-GH was broadened (37-43℃) under the regulation of heat resistance device gapA-T.te-HSP20. Stress resistance analysis showed that E. coli-GH not only possessed heat resistance and butanol resistance

but also had some resistance to acetic acid and ethanol. These results provide a new idea for modern microorganisms industry.

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