Salting-out extraction of phenolics from grape (Vitis vinifera) seed

DANG Yanyan; GUO Yuxi; CAO Wenjing; XIU Zhilong

doi:10.3969/j.issn.0438-1157.2014.08.028

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Salting-out extraction of phenolics from grape (Vitis vinifera) seed

更新时间：2025-12-10

- Salting-out extraction of phenolics from grape (Vitis vinifera) seed
- CIESC Journal Issue 8, Pages: 3048-3053(2014)
- 作者机构：
  
  1. 大连理工大学生命科学与技术学院,辽宁,大连,116024
  2. 石河子大学化学化工学院,新疆,石河子,832003
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2014.08.028
  CLC： Q464.9
- Online First：05 August 2014，
  
  Published：05 August 2014
- 稿件说明：
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DANG Yanyan, GUO Yuxi, CAO Wenjing, et al. Salting-out extraction of phenolics from grape (Vitis vinifera) seed[J]. CIESC Journal, 2014, (8): 3048-3053.
DOI：

DANG Yanyan, GUO Yuxi, CAO Wenjing, et al. Salting-out extraction of phenolics from grape (Vitis vinifera) seed[J]. CIESC Journal, 2014, (8): 3048-3053. DOI： 10.3969/j.issn.0438-1157.2014.08.028.

摘要

采用乙醇/（NH4）2SO4两相盐析体系，从酿酒副产物葡萄籽中提取多酚、类黄酮和原花青素。实验结果表明，在室温下用组成（质量分数）为30%乙醇/18%（NH4）2SO4的体系提取葡萄籽中多酚、类黄酮以及原花青素，得率分别为64.54、42.45、23.16 mg·g-1；它们富集于上相乙醇相，萃取回收率分别为97.31%、98.19%、97.92%。室温条件下用2，2-二苯基-1-苦基肼（DPPH）法与2，2'-连氮基-双-（3-乙基苯并二氢噻唑啉-6-磺酸）（ABTS）法测定两相盐析萃取物抗氧化、清除自由基能力，IC50分别为61.85、71.76 mg·L-1，比传统的热回流提取物具有更强的抗氧化活性。

Abstract

Salting-out extraction system composed of ethanol and ammonium sulfate was used to separate total phenolics

flavonoid

and proanthocyanidin from wine grape seed. The experimental results showed that components were extracted into the top ethanol-rich phase (the system consisted of 30% (mass) ethanol/18% (mass) ammonium sulfate) to give the yield of total phenolics

flavonoid

and proanthocyanidin in 64.54

42.45

and 23.16 mg·g-1

and corresponding recovery rates in 97.31%

98.19%

and 97.92%

respectively. The antioxidant activity of seed extracts were assayed with radical-scavenging ability (IC50) of 2

2-diphenyl-1-picrylhydrazyl and 2

2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at room temperature. The seed extracts by salting-out extraction showed lower IC50 (61.85 and 71.76 mg·L-1) than that by heat reflux extraction (78.73 and 90.34 mg·L-1).

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