Mesoporous TiO<sub>2</sub> with different selectivities of protein immobilization performance

DONG Yihui; AN Rong; ZHUANG Wei; YAO Zhong; ZHU Yudan; LIU Chang; LU Xiaohua

doi:10.3969/j.issn.0438-1157.2014.05.027

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Mesoporous TiO₂ with different selectivities of protein immobilization performance

更新时间：2025-12-10

- Mesoporous TiO₂ with different selectivities of protein immobilization performance
- CIESC Journal Issue 5, (2014)
- 作者机构：
  
  1. 南京工业大学材料化学工程国家重点实验室,江苏,南京,210009
  2. Department of Chemical and Biomolecular Engineering, North Carolina State University,NC,Raleigh,USA,27695-7905
  3. 南京工业大学生物与制药学院,江苏,南京,211816
  4. 南京工业大学食品与轻工学院,江苏,南京,210009
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2014.05.027
  CLC： 647.32TB383
- Online First：05 May 2014，
  
  Published：05 May 2014
- 稿件说明：
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DONG Yihui, AN Rong, ZHUANG Wei, et al. Mesoporous TiO₂ with different selectivities of protein immobilization performance[J]. CIESC Journal, 2014, (5). DOI: 10.3969/j.issn.0438-1157.2014.05.027.
DOI：

DONG Yihui, AN Rong, ZHUANG Wei, et al. Mesoporous TiO₂ with different selectivities of protein immobilization performance[J]. CIESC Journal, 2014, (5). DOI: 10.3969/j.issn.0438-1157.2014.05.027. DOI：

摘要

通过500℃下焙烧H2Ti2O5制备介孔TiO2-500作为高效液相色谱的固定相填料，测试牛血清蛋白（BSA）、肌红蛋白（myoglobin）、溶菌酶（lysozyme）在不同pH环境下的停留时间分布，结果表明，在pH=4.7，三种蛋白质几乎都固定化，在pH=7.4和11条件下，三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO2-500表面的宏观常压吸附量的结果与色谱结果相一致，介孔TiO2-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此，该介孔TiO2对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。

Abstract

Mesoporous TiO2 with adjustable pore diameter

high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO2-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H2Ti2O5 at 500℃

and the TiO2 samples were characterized by X-ray diffraction (XRD)

N2 adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence time under different pH of bovine serum albumin

myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7

and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO2-500 under atmospheric pressure was consistent with chromatographic results

and the order of the immobilization capacity of mesoporous TiO2-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore

due to the significant difference in protein immobilization performance

the mesoporous TiO2 has a potential application in protein adsorption and separation

immobilizing enzyme and other fields.

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Mesoporous TiO2 with different selectivities of protein immobilization performance

DOI：10.3969/j.issn.0438-1157.2014.05.027

摘要

Abstract

关键词

Keywords

references

Mesoporous TiO₂ with different selectivities of protein immobilization performance