Combining hydrophobic charge-induction chromatography (HCIC) and expanded bed adsorption (EBA)

a novel efficient bioseparation process was developed to purify the immunoglobulin IgY from poultry blood. New hydrophobic charge-induction expanded bed adsorbent S-MMI was prepared with 2-mercapto-1-methyl imidazole (MMI) as the HCIC ligands coupled to quartz-densified agarose matrix for EBA. S-MMI had high ligand density (about 105 mmol·g-1) and the saturated adsorption capacity for IgY reached 71.26 mg·ml-1 at pH 7. S-MMI could form perfectly classified fluidization in expanded bed with high stability. Chicken plasma was precipitated with caprylic acid and the supernatant was used as the feedstock for IgY separation. The loading and elution conditions were optimized by packed bed chromatography. The effects of expansion factors in expanded bed were investigated and the separation conditions were optimized. Finally

IgY was purified from pretreated chicken plasma by EBA with high purity of 98.9% and yield of 80.5%. The combination of HCIC and EBA could achieve efficient separation of immunoglobulin from crude feedstock

which provided a new way for the comprehensive utilization of poultry blood resource.