Consecutive batch fermentation with self-flocculating yeast realized recycling of cell and increased ethanol yield and productivity.Oxidoreduction potential(ORP)control was used in this process to automatically feed the fresh medium at the end of every batch by judging the uprising of ORP curve.When ORP value was controlled at-100 mV and initial glucose were chosen as 200 g·L-1(industrial concentration)and 250 g·L-1(very high gravity)
compared with batch fermentation
accumulated biomass in consecutive batch sharply declined by 42.4%(~200 g·L-1
5 batches)and 16.3%(~250 g·L-1
6 batches)
respectively.On the other hand
compared with manual consecutive batch fermentation
automatic process greatly shortened fermentation time by 17.8%(~200 g·L-1)and 15.2%(~250 g·L-1).Consequently
ethanol productivities were enhanced by 21.7% and 17.9%