Effects of ions and surfactant on enzymatic hydrolysis of sweet sorghum bagasse

WANG Wen; ZHUANG Xinshu; YUAN Zhenhong; YU Qiang; QI Wei; WANG Qiong; TAN Xuesong

doi:10.3969/j.issn.0438-1157.2013.10.038

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Effects of ions and surfactant on enzymatic hydrolysis of sweet sorghum bagasse

更新时间：2025-12-10

- Effects of ions and surfactant on enzymatic hydrolysis of sweet sorghum bagasse
- CIESC Journal Issue 10, Pages: 3767-3774(2013)
- 作者机构：
  
  1. 中国科学院广州能源研究所, 中国科学院可再生能源重点实验室,广东,广州,510640
  2. 中国科学院研究生院,北京,100039
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.10.038
  CLC： 216.2Q814.9
- Online First：05 October 2013，
  
  Published：05 October 2013
- 稿件说明：
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WANG Wen, ZHUANG Xinshu, YUAN Zhenhong, et al. Effects of ions and surfactant on enzymatic hydrolysis of sweet sorghum bagasse[J]. CIESC Journal, 2013, (10): 3767-3774.
DOI：

WANG Wen, ZHUANG Xinshu, YUAN Zhenhong, et al. Effects of ions and surfactant on enzymatic hydrolysis of sweet sorghum bagasse[J]. CIESC Journal, 2013, (10): 3767-3774. DOI： 10.3969/j.issn.0438-1157.2013.10.038.

摘要

为了提高纤维素酶水解经高温液态水处理后的甜高粱秆渣的效率

探讨了多种阴离子、阳离子以及吐温80(Tween 80)对纤维素酶活力的影响

并初步探讨了Tween 80影响甜高粱秆渣酶解的机制。酶激活试验表明

Br-、I-、NO3-、Ca2+、Mg2+和Co2+对纤维素酶有激活作用

但对甜高粱秆渣的水解效率提高不明显。添加Tween 80发现

随着浓度的增加

它对纤维素酶的抑制作用增强

而Tween 80添加量为0.175 ml·(g甜高粱秆渣)-1时

甜高粱秆渣的酶解效率由16.6%提高到37.9%。吸附试验表明

甜高粱秆渣对纤维素酶和Tween 80的吸附达到一定限度后不再上升

Tween 80能显著降低甜高粱秆渣对纤维素酶的吸附。红外光谱分析发现

木质素对Tween 80的吸附要强于它对纤维素酶的吸附。

Abstract

In order to enhance the enzymatic hydrolysis effectiveness of sweet sorghum bagasse(SSB)pretreated by liquid hot water(LHW)

the effects of anions

cations and Tween 80 on hydrolysis activity of enzyme and acting mechanism of Tween 80 were investigated.The tests of hydrolysis activity showed that Br-

I-

NO3-

Ca2+

Mg2+ and Co2+ could activate cellulase

but had little improvement on enzymatic hydrolysis of SSB.0.175 ml·(g dry material)-1 of Tween 80 could make enzymatic hydrolytic efficiency of SSB increase from 16.6% to 37.9%

but higher concentration of Tween 80 would inhibit the enzyme activity

and thus hinder the enzymatic hydrolyzing process.Adsorption experiments showed that there was limit adsorption of Tween 80 and cellulase on SSB.Tween 80 could obviously reduce the adsorption of cellulase on SSB.By FTIR measurement

it showed that the adsorption of Tween 80 was stronger on lignin than on cellulase.

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