Triphenyltin biodegradation and differential expression proteins of degrading bacteria

XIAO Qiaoqiao; YE Jinshao; YIN Hua; CHEN Shuona; PENG Hui; A Runa

doi:10.3969/j.issn.0438-1157.2013.10.034

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Triphenyltin biodegradation and differential expression proteins of degrading bacteria

更新时间：2025-12-10

- Triphenyltin biodegradation and differential expression proteins of degrading bacteria
- CIESC Journal Issue 10, Pages: 3732-3740(2013)
- 作者机构：
  
  1. 暨南大学环境工程系, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广东,广州,510632
  2. 华南理工大学环境与能源学院, 工业聚集区污染控制与生态修复教育部重点实验室,广东,广州,510006
  3. 东北电力大学环境工程系,吉林市,吉林省,132012
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.10.034
  CLC： 703.1
- Online First：05 October 2013，
  
  Published：05 October 2013
- 稿件说明：
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XIAO Qiaoqiao, YE Jinshao, YIN Hua, et al. Triphenyltin biodegradation and differential expression proteins of degrading bacteria[J]. CIESC Journal, 2013, (10): 3732-3740.
DOI：

XIAO Qiaoqiao, YE Jinshao, YIN Hua, et al. Triphenyltin biodegradation and differential expression proteins of degrading bacteria[J]. CIESC Journal, 2013, (10): 3732-3740. DOI： 10.3969/j.issn.0438-1157.2013.10.034.

摘要

考察了克雷伯氏菌(Klebsiella pneumoniae)对浓度为0.1～10 mg·L-1的三苯基锡(TPhT)的生物降解

分析了在无机盐培养基和去离子水中K.pneumoniae与TPhT作用过程中

其生理特性和蛋白组的变化

以期从降解菌蛋白质组以及菌体离子代谢的角度揭示有机锡的微生物降解机理。实验结果显示K.pneumoniae能有效地将TPhT降解为二苯基锡、一苯基锡和锡。TPhT在无机盐培养基和去离子水中的降解率分别为46.7%～73.6%和25.1%～87.0%。在此过程中TPhT增加了菌体细胞膜的通透性

从而引发细胞膜的损害

刺激了细胞内离子的释放

造成部分细胞的死亡。TPhT诱导菌体表达了部分与蛋白质水解、代谢过程、蛋白质运输、能量转换、TPhT抗性和降解有关的差异蛋白。

Abstract

To investigate the mechanism of triphenyltin(TPhT)biodegradation through proteomic and ions release

the biodegradation of TPhT with concentration from 0.1 to 10 mg·L-1 by Klebsiella pneumoniae

and the physiological responses and proteomic changes of K.pneumoniae to TPhT exposure in mineral salts medium(MSM)and deionized distilled water(DDW)were studied.Experimental results showed that K.pneumonia could effectively degrade TPhT to diphenyltin(DPhT)

monophenyltin(MPhT)and tin(Sn)

and increase TPhT removal rate from 46.7% to 73.6% and from 25.1% to 87.0% in MSM and DDW

respectively.In this process

TPhT activated membrane permeability and triggered cell membrane damage

which increased the release of intracellular ions and caused part of cell death. Moreover

the exposure of K.pneumonia to TPhT induced differential expression of some proteins that were responsible for proteolysis

metabolic processes

protein translocation

energy conversion

TPhT stress tolerance

and TPhT elimination.

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