Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

LI Weijie; ZHOU Xinli; LIU Baolin; LÜ Fukou

doi:10.3969/j.issn.0438-1157.2013.08.038

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Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

更新时间：2025-12-10

- Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes
- CIESC Journal Issue 8, Pages: 2969-2974(2013)
- 作者机构：
  
  上海理工大学生物热科学研究所,上海,200093
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.08.038
  CLC： K6Q599
- Online First：05 August 2013，
  
  Published：05 August 2013
- 稿件说明：
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LI Weijie, ZHOU Xinli, LIU Baolin, et al. Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes[J]. CIESC Journal, 2013, (8): 2969-2974.
DOI：

LI Weijie, ZHOU Xinli, LIU Baolin, et al. Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes[J]. CIESC Journal, 2013, (8): 2969-2974. DOI： 10.3969/j.issn.0438-1157.2013.08.038.

摘要

Abstract

When cells are cryopreserved

the morphology of cryoprotectant at crystallization and recrystallization would influence the survival rate of cells.The cryo-microscope system was used to observe the morphology of crystal and sizes of two cryoprotectants during cooling and rewarming at different speeds.It is found that crystals during cooling are smaller and less at higher speed than at lower speed

and the former is favorable for preservation of cells.When the vitrified cryoprotetant is rewarmed

crystals during recrystallization are smaller and less at higher speed than at lower speed

and the former with narrow danger zone is favorable for the survival of oocytes.Besides

high concentration of cryoprotectant is more favorable for the survivalrate of oocytes than lower concentration.

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