Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO<sub>2</sub> whiskers

YE Lijing; FANG Xin; WANG Haoqi; YAO Zhong; XIONG Qiang; ZHOU Zhi; SUN Yun; WEI Ping

doi:10.3969/j.issn.0438-1157.2013.06.035

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Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers

更新时间：2025-12-10

- Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers
- CIESC Journal Issue 6, Pages: 2160-2168(2013)
- 作者机构：
  
  南京工业大学食品与轻工学院,江苏,南京,210009
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.06.035
  CLC： 814
- Online First：05 June 2013，
  
  Published：05 June 2013
- 稿件说明：
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YE Lijing, FANG Xin, WANG Haoqi, et al. Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers[J]. CIESC Journal, 2013, (6): 2160-2168. DOI: 10.3969/j.issn.0438-1157.2013.06.035.
DOI：

YE Lijing, FANG Xin, WANG Haoqi, et al. Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers[J]. CIESC Journal, 2013, (6): 2160-2168. DOI: 10.3969/j.issn.0438-1157.2013.06.035. DOI：

摘要

以3-氨丙基三乙氧基硅烷(APTES)对介孔二氧化钛晶须(MTiO2_ws)进行表面修饰

使其电荷零点值(pzc)由5.3提高至6.8

改性后材料MTiO2_ws-APTES的比表面积略有上升

但孔结构基本不变。以MTiO2_ws-APTES为载体对γ-谷氨酰转肽酶(GGT)进行了固定化

当给酶量小于150 U·g-1时

酶活回收率均大于99%

固定化酶MTiO2_ws-APTES-GGT的比活力最高可达184.0 U·g-1

对酶的负载性能显著优于MTiO2_ws。相比于游离酶

MTiO2_ws-APTES-GGT的最适温度和热稳定性均略有下降

但pH稳定性明显优于游离酶和以MTiO2_ws为载体的固定化酶(MTiO2_ws-GGT); MTiO2_ws-APTES-GGT对γ-谷氨酰对硝基苯胺(GpNA)的亲和力常数(Km)为0.889 mmol·L-1

较游离酶有所上升

但小于MTiO2_ws-GGT。MTiO2_ws-APTES-GGT的稳定性良好

经4℃下储藏60 d

连续使用21批次后残余酶活仍可达初始值的80.07%。

Abstract

Mesoporous TiO2 whiskers (MTiO2_ws) were chemically modified with 3-aminopropyltriethyloxy silane (APTES).As a result

the point of zero charge (pzc) for the modified material (MTiO2_ws-APTES) rose from 5.3 to 6.8.Also the specific surface area of MTiO2_ws-APTES slightly increased

but the pore structure had little change compared to that of MTiO2_ws.Then

MTiO2_ws-APTES was used as the support for γ-glutamyltranspeptidase (GGT) immobilization.MTiO2_ws-APTES showed better loading capacity than MTiO2_ws.While the enzyme concentration was less than 150 U·g-1

the recovery of activity was above 99% and the highest specific activity for immobilized GGT (MTiO2_ws-APTES-GGT) was 184.0 U·g-1.Although both optimal temperature and thermal stability of MTiO2_ ws-APTES-GGT slightly decreased compared with the native enzyme

pH stability was greatly improved.The affinity constant (Km) of MTiO2_ws-APTES-GGT toward γ-glutamyl-p-nitroaniline (GpNA) was also determined to be 0.889 mmol·L-1

which was higher than that of the native enzyme

but less than that of MTiO2_ws-GGT.After storage at 4℃ for 60 days and reused for 21 batches

MTiO2_ws-APTES-GGT maintained 80.07% of its initial activity.

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Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO2 whiskers

DOI：10.3969/j.issn.0438-1157.2013.06.035

摘要

Abstract

关键词

Keywords

references

Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO₂ whiskers