Molecular identification and conditions for preliminary application of a Bacillus sp.

WU Xiang; QU Yi; CHENG Guojun; NIE Wenchao; DU Dongyun

doi:10.3969/j.issn.0438-1157.2013.04.046

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Molecular identification and conditions for preliminary application of a Bacillus sp.

更新时间：2025-12-10

- Molecular identification and conditions for preliminary application of a Bacillus sp.
- CIESC Journal Issue 4, Pages: 1459-1465(2013)
- 作者机构：
  
  1. 中南民族大学环境科学与工程研究所,中南民族大学化学与材料科学学院/催化材料科学国家民委-教育部共建重点实验室,湖北,武汉,430074
  2. 中南民族大学生命科学学院,湖北,武汉,430074
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.04.046
  CLC： 93
- Online First：05 April 2013，
  
  Published：05 April 2013
- 稿件说明：
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WU Xiang, QU Yi, CHENG Guojun, et al. Molecular identification and conditions for preliminary application of a Bacillus sp.[J]. CIESC Journal, 2013, (4): 1459-1465.
DOI：

WU Xiang, QU Yi, CHENG Guojun, et al. Molecular identification and conditions for preliminary application of a Bacillus sp.[J]. CIESC Journal, 2013, (4): 1459-1465. DOI： 10.3969/j.issn.0438-1157.2013.04.046.

摘要

Abstract

Ethyl chloride is an important intermediate of pesticide.It is widely used to produce organic phosphorus pesticide with high effect and low toxicity.Toxic wastewater produced in the manufacture process contains high values of chemical oxygen demand

total phosphorus

and sulfur ion.A bacterial strain SCUN which could aerobically degrade ethyl chloride wastewater was isolated from the natural water body near the wastewater discharge facility.This strain was identified as Bacillus sp.SCUN.based on its morphological observation and 16S rDNA sequence analysis.Both indole and hydrogen sulfide were not detected in the experiments of IMViC.Salt tolerance of the microorganism could reach 7%.The condition for its industrial application was discussed.It was found that at pH value of 7.0

immobilized microorganism ratio of 5%

temperature of 30℃

the microorganism showed the best growth for biodegradation.With a newly developed ternary cycle treatment

the COD removal rate approached 58.3%.

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