To prepare the cellulose-based chromatography matrix with uniform size

a cross-flow microchannel chip was used to control the droplet size of cellulose solution and then the microdroplets were solidified to obtain the monodisperse cellulose beads.In the present work

ionic liquid

[EMIM]MP

was used to dissolve the microcrystalline cellulose as water phase and sunflower oil was used as oil phase.The effects of cellulose concentration

surfactant addition

flow rates of oil phase and water phase were investigated.The optimized preparation condition was 2% cellulose solution as water phase

flow rate of water phase of 6 μl·min-1 and flow rate of oil phase of 200 μl·min-1.The microdroplets of cellulose solution thus obtained could be about 100 μm with the variation coefficient less than 0.2.After solidification and regeneration

cellulose beads were obtained with good sphericity.Wet density of beads was 1.019 g·ml-1

porosity was 94.6%

and mean size was about 105.5 μm.The cellulose beads were coupled with DEAE ligand and used as weak anion-exchange resin.The ion exchange capacity was 123.3 μmol·g-1.Static adsorption and adsorption kinetics were studied.The results showed that saturated adsorption capacity for bovine serum albumin could reach 220 mg·g-1

and the effective diffusion coefficient was 1.8×10-11 m2·s-1

which showed potential application for protein separation.