The mechanical model of fed-batch penicillin fermentation has been well studied for the last decades

but most of them can hardly be implemented to control fermentation process.Due to this problem

this paper uses Birol’s unstructured model

adjusts temperature and pH dynamics of the fermentation and deduces a simplified penicillin unstructured model.To increase production of penicillin

substrate feedrate which is critical in fermentation process is optimized.Because of the nonlinearity and constraints of the mechanical model

sequential quadratic programming(SQP)algorithm is used to the whole process

in which the feedrate trajectory is divided equally into several intervals to enhance optimization efficiency.Optimization results show that the penicillin concentration and yield are increased compared to the normal input.