Characteristics of simultaneous nitrification and denitrification <italic style="font-style: italic">via</italic> nitrite and N<sub>2</sub>O emission in SBBR under different DO concentrations

GONG Youkui; REN Lifang; PENG Yongzhen

doi:10.11949/j.issn.0438-1157.20181333

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Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations

Energy and environmental engineering | 更新时间：2026-07-24

- Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations
- CIESC Journal Vol. 70, Issue 4, Pages: 1550-1558(2019)
- 作者机构：
  
  1.烟台职业学院建筑工程系，山东烟台 264000
  2.北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20181333
  CLC： X 703.1
- Received：15 November 2018，
  
  Revised：2018-12-08，
  
  Online First：15 June 2020，
  
  Published：12 April 2019
- 稿件说明：
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巩有奎, 任丽芳, 彭永臻. 不同DO下SBBR亚硝酸型同步脱氮及N₂O释放特性[J]. 化工学报, 2019, 70(4): 1550-1558 DOI: 10.11949/j.issn.0438-1157.20181333.

GONG Youkui, REN Lifang, PENG Yongzhen. Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations[J]. CIESC Journal, 2019, 70(4): 1550-1558 DOI: 10.11949/j.issn.0438-1157.20181333.
巩有奎, 任丽芳, 彭永臻. 不同DO下SBBR亚硝酸型同步脱氮及N₂O释放特性[J]. 化工学报, 2019, 70(4): 1550-1558 DOI: 10.11949/j.issn.0438-1157.20181333. DOI：

GONG Youkui, REN Lifang, PENG Yongzhen. Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations[J]. CIESC Journal, 2019, 70(4): 1550-1558 DOI: 10.11949/j.issn.0438-1157.20181333. DOI：

摘要

在(20±2.0)℃ 条件下，以实际生活污水为处理对象，以碳纤维为填料（填充率35%），利用序批式生物膜（sequencing batch biofilm reactor，SBBR）反应器，通过限氧曝气，成功实现了亚硝酸型同步生物脱氮（simultaneous nitrification and denitrification，SND）过程。荧光原位杂交技术（fluorescence

situ

hybridization，FISH）半定量表明，氨氧化菌（ammonia oxidizing bacteria

AOB）是硝化系统中的优势菌种。微生物将外碳源以聚

–羟基烷酸酯(poly-

-hydroxyalkanoate，PHA)的形式储存在体内，作为后续反硝化过程所需内碳源。DO=0.5 mg/L，SBBR系统NH

-N和TN去除率分别为95%以上和80.4%，SND效率达77.9%。出水NO

-N小于10 mg/L，且以NO

-N为主。DO=2.0、1.2和0.5 mg/L时，系统N

O释放量分别为1.38、2.39和1.65 mg/L。AOB的好氧反硝化过程和低氧条件下以PHA作为内碳源的NO

-N反硝化过程，都会导致N

O释放。低DO水平是

实现亚硝酸型同步脱氮过程和降低N

O释放的关键因素。低DO促进了AOB的竞争优势，形成了良好的缺氧微环境，降低了COD降解速率，为反硝化过程提供外碳源作为电子供体，从而降低了N

O释放量。

Abstract

At normal temperature of (20±2.0)℃

using actual domestic wastewater as influent substrate and carbon fibre as biological carriers (filling rate 35%)

the simultaneous nitrification and denitrification

via

nitrite was achieved in a sequencing batch biofilm reactor under limited DO concentrations. The results of fluorescence

situ

hybridization (FISH) demonstrated that the ammonia oxidizing bacteria (AOB) became the dominant species in the nitrification process under limited DO concentration. During the anoxic and the initial aerobic process

the external COD was taken up and converted to polyhydroxyalkanoate (PHA)

which can be used as internal carbon sources for the following denitrification. DO=0.5 mg/L

the NH

-N removal

TN removal and simultaneous nitrification and denitrification efficiency was more than 95%

80.4% and 77.9%

respectively. The concentration of NO

-N in the effluent was less than 10 mg/L and mainly in the form of NO

-N. When the DO concentration was controlled at 2.0

1.2 and 0.5 mg/L

the N

O emission was 1.38

2.39 and 1.65 mg/L

respectively. Both the aerobic denitrification process of AOB and the denitrification of NO

-N with PHA as carbon source at the presence of lower oxygen can lead to the release of N

O. The long time of lower DO conditions promoted AOB s competitive advantage

formed a micro-environment for denitrifiers

reduced the COD degradation rate

and provided an external carbon source as an electronic donor for denitrification

thereby reducing N

O emissions.

关键词

Keywords

references

Wang J , Rong H , Zhang C . Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor [J ] . Journal of Bioscience and Bioengineering , 2018 , 125 ( 5 ): 532 - 542 .

Zhao J , Feng L , Yang G , et al . Development of simultaneous nitrification- denitrification (SND) in biofilm reactors with partially coupled a novel biodegradable carrier for nitrogen-rich water purification [J ] . Bioresource Technology , 2017 , 243 : 800 - 809 .

Han Y , Liu J , Guo X , et al . Micro-environment characteristics and microbial communities in activated sludge flocs of different particle size [J ] . Bioresource Technology , 2012 , 124 ( 11 ): 252 - 258 .

Ma W , Han Y , Ma W , et al . Enhanced nitrogen removal from coal gasification wastewater by simultaneous nitrification and denitrification (SND) in an oxygen-limited aeration sequencing batch biofilm reactor [J ] . Bioresource Technology , 2017 , 44 ( 11 ): 84 - 91 .

Leonidia M C D , Eloisa P , Eugenio F , et al . Removal of ammonium via simultaneous nitrification-denitrification nitrite-shortcut in a single packed-bed batch reactor [J ] . Bioresource Technology , 2009 , 100 ( 3 ): 1100 - 1107 ．

Stefano M , Giaime T , Giovannimatteo E . Evaluation of nitrous oxide gaseous emissions from a partial nitritation reactor operating under different conditions [J ] . Desalination and Water Treatment , 2018 , DOI: 10.5004/dwt.2018.22934 http://dx.doi.org/10.5004/dwt.2018.22934 .

Wen Q , Chen Z , Shi H . T-RFLP detection of nitrifying bacteria in a fluidized bed reactor of achieving simultaneous nitrification- denitrification .[J ] . Chemosphere , 2008 , 71 ( 9 ): 1683 - 1692 .

Solomon S , Qin D , Manning M , et al . Climate Change 2007: the Physical Science Basis [M ] . Cambridge : Cambridge University Press , 2007 .

Ravishankara A R , Daniel J S , Portmann R W . Nitrous oxide(N 2 O): the dominant ozone-depleting substance emitted in the 21st century [J ] . Science , 2009 , 326 ( 5949 ): 123 - 125 .

Kong Q , Wang Z , Niu P , et al . Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process [J ] . Bioresource Technology , 2016 , 210 : 94 - 100 .

Ye L , Ni B J , Law Y Y , et al . A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators [J ] . Water Research , 2014 , 48 ( 1 ): 257 - 268 .

巩有奎 , 王赛 , 彭永臻 , 等 . 生活污水不同生物脱氮过程中N 2 O产量及控制 [J ] . 化工学报 , 2010 , 61 ( 5 ): 1286 - 1292 .

Gong Y K , Wang S , Peng Y Z , et al . Formation of N 2 O in various biological nitrogen removal processes for treatment of domestic sewage [J ] . CIESC Journal , 2010 , 61 ( 5 ): 1286 - 1292 .

APHA(American Public Health Association) . Standard methods for the examination of water and wastewater [S ] . Baltimore : Port City Press , 1998 .

Oehmen A , Keller-Lehmann B , Zeng R J , et al . Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J ] . Journal of Chromatography A , 2005 , 1070 ( 1/2 ): 131 - 136 .

张建华 , 彭永臻 , 张淼 , 等 . 同步硝化反硝化SBBR 处理低C/N 比生活污水的启动与稳定运行 [J ] . 化工学报 , 2016 , 67 ( 11 ): 4817 - 4824 .

Zhang J H , Peng Y Z , Zhang M , et al . Start-up and steady operation of simultaneous nitrification and denitrification in SBBR treating low C/N ratio domestic wastewater [J ] . CIESC Journal , 2016 , 67 ( 11 ): 4817 - 4824 .

曾薇 , 杨庆 , 张树军 , 等 . 采用 FISH、DGGE 和 Cloning 对短程脱氮系统中硝化菌群的比较分析 [J ] . 环境科学学报 , 2006 , 26 ( 5 ): 734 - 739 .

Zeng W , Yang Q , Zhang S J , et al . Analysis of nitrifying bacteria in short-cut nitrification- denitrification processes by using FISH, PCR-DGGE and Cloning [J ] . Acta Scientiae Circumstantiae , 2006 , 26 ( 5 ): 734 - 739 .

王建龙 , 吴立波 , 齐星 , 等 . 用氧吸收速率( OUR) 表征活性污泥硝化活性的研究 [J ] . 环境科学学报 , 1999 , 19 ( 3 ): 225 - 229 .

Wang J L , Wu L B , Qi X , et al . Characterization of nitrification activity of activated sludge by oxygen uptake rate (OUR) [J ] . Acta Scientiae Circumstantiae , 1999 , 19 ( 3 ): 225 - 229 .

巩有奎 , 苗志加 , 彭永臻 . 生物脱氮好氧阶段不同反应过程N 2 O产量 [J ] . 环境工程学报 , 2016 , 12 ( 12 ): 6963 - 6968 .

Gong Y K , Miao Z J , Peng Y Z . Nitrous oxide production from sequencing batch reactor sludge under nitrifying conditions of different process [J ] . Chinese Journal of Environmental Engineering , 2016 , 12 ( 12 ): 6963 - 6968 .

Yilmaz G , Lemaire R , Keller J , et al . Simultaneous nitrification, denitrification, and phosphorus removal from nutrient-rich industrial wastewater using granular sludge [J ] . Biotechnol. Bioeng. , 2008 , 100 ( 3 ): 529 - 541 .

Rikke L M , Zeng R L , Giugliano V , et al . Challenges for simultaneous nitrification, denitrification, and phosphorus removal in microbial aggregates: mass transfer limitation and nitrous oxide production [J ] . FEMS Microbiology Ecology , 2005 , 52 ( 3 ): 329 - 338 .

Guisasola A , Jubany I , Baeza J A , et al . Respirometric estimation of the oxygen affinity constants for biological ammonia and nitrite oxidation [J ] . Journal of Chemical Technology & Biotechnology , 2005 , 80 ( 4 ): 388 - 396 .

Soliman M , Eldyasti A . Ammonia-oxidizing bacteria (AOB): opportunities and applications—a review [J ] . Reviews in Environmental Science and Bio/Technology , 2018 , DOI: 10.1007/s11157-018-9463-4 http://dx.doi.org/10.1007/s11157-018-9463-4 .

Bernet N , Dangcong P , Delgenes J P , et al . Nitrification at low oxygen concentration in biofilm reactor [J ] . Journal of Environmental Engineering-ASCE , 2001 , 127 ( 3 ): 266 - 271 .

Laanbroek H J , Gerards S . Competition for limiting amounts of oxygen between Nitrosomonas europaea and Nitrobacter winogradskyi grown in mixed continuous cultures [J ] . Archives of Microbiology , 1993 , 159 ( 5 ): 453 - 459 .

曾薇 , 张悦 , 李磊 . 限氧曝气实现亚硝酸型同步硝化反硝化的研究 [J ] . 北京工业大学学报 , 2010 , 36 ( 9 ): 1263 - 1270 .

Zeng W , Zhang Y , Li L . Simultaneous nitrification denitrification via nitrite under limited aeration [J ] . Journal of Beijing University of Technology , 2010 , 36 ( 9 ): 1263 - 1270 .

Law Y , Ye L , Pan Y , et al . Nitrous oxide emissions from wastewater treatment processes [J ] . Philosophical Transactions of the Royal Society B: Biological Sciences , 2012 , 367 ( 1593 ): 1265 - 1277 .

Yang Q , Liu X H , Peng C Y , et al . N 2 O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method [J ] . Environmental Science & Technology , 2009 , 43 ( 24 ): 9400 - 9406 .

Pan Y T , Ni B J , Yuan Z G . Modeling electron competition among nitrogen oxides reduction and N 2 O accumulation in denitrification [J ] . Environmental Science & Technology , 2013 , 47 ( 19 ): 11083 - 11091 .

巩有奎 , 王淑莹 , 王莎莎 . 碳氮比对短程反硝化过程中N 2 O产生的影响 [J ] . 化工学报 , 2011 , 62 ( 7 ): 2049 - 2054 .

Gong Y K , Wang S Y , Wang S S . Effect of C/N ratio on N 2 O accumulation and reduction during nitrite denitrification process [J ] . CIESC Journal , 2011 , 62 ( 7 ): 2049 - 2054 .

Pan Y T , Ye L , Ni J B , et al . Effect of pH on N 2 O reduction and accumulation during denitrification by methanol utilizing denitrifiers [J ] . Water Research , 2012 , 46 ( 15 ): 4832 - 4840 .

Zhao W , Wang Y , Liu S , et al . Denitrification activities and N 2 O production under salt stress with varying CODN ratios and terminal electron acceptors [J ] . Chemical Engineering Journal , 2013, 215 / 216 ( 2 ): 252 - 260 .

Gong Y , Peng Y , Yang Y . Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate [J ] . Journal of Hazardous Materials , 2012 , 227/228 : 453 - 460 .

Pan Y , Ni B , Lu H , et al . Evaluating two concepts for the modelling of intermediates accumulation during biological denitrification in wastewater treatment [J ] . Water Research , 2015 , 71 ( 15 ): 21 - 31 .

Chen X , Yuan Z , Ni B J . Nitrite accumulation inside sludge flocs significantly influencing nitrous oxide production by ammonium-oxidizing bacteria [J ] . Water Research , 2018 , 143 ( 15 ): 99 - 108 .

郑桂林 , 张朝升 , 曹勇锋 , 等 . DO在SBBR生物膜中传递及对同步硝化反硝化的影响 [J ] . 中国给水排水 , 2016 , 32 ( 3 ): 22 - 26 .

Zheng G L , Zhang C S , Cao Y F , et al . Transfer of DO in biofilm and effect of DO on simultaneous nitrification and denitrification in sequencing batch biofilm reactor [J ] . China Water＆Wastewater , 2016 , 32 ( 3 ): 22 - 26 .

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Characteristics of simultaneous nitrification and denitrification via nitrite and N2O emission in SBBR under different DO concentrations

DOI：10.11949/j.issn.0438-1157.20181333

摘要

Abstract

关键词

Keywords

references

Characteristics of simultaneous nitrification and denitrification via nitrite and N₂O emission in SBBR under different DO concentrations