Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells

TANG Cunduo; SHI Hongling; HE Zihan; DING Pengju; JIAO Zhujin; KAN Yunchao; YAO Lunguang

doi:10.11949/j.issn.0438-1157.20171414

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Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells

更新时间：2025-12-10

- Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells
- CIESC Journal Issue 6, Pages: 2627-2631(2018)
- 作者机构：
  
  1. 南阳师范学院昆虫生物反应器河南省工程实验室和河南省南水北调中线水源区生态安全重点实验室,河南,南阳,473061
  2. 华东理工大学生物反应器工程国家重点实验室,上海,200237
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20171414
  CLC： Q92TQ317
- Online First：05 June 2018，
  
  Published：05 June 2018
- 稿件说明：
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TANG Cunduo, SHI Hongling, HE Zihan, et al. Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells[J]. CIESC Journal, 2018, (6): 2627-2631.
DOI：

TANG Cunduo, SHI Hongling, HE Zihan, et al. Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells[J]. CIESC Journal, 2018, (6): 2627-2631. DOI： 10.11949/j.issn.0438-1157.20171414.

摘要

苯乙酮酸是化学合成中重要的合成砌块，可用于合成多种药物中间体，探索苯乙酮酸的绿色合成工艺具有重要的意义。以包含D-扁桃酸脱氢酶LhDMDH编码基因的重组大肠杆菌全细胞为催化剂，考察了它在无辅酶和辅底物添加的条件下对D-扁桃酸生物转化的效果，并对催化产物进行了纯化和鉴定。结果表明，本研究成功实现了在无辅酶和辅底物添加条件下苯乙酮酸的生物合成，产物的得率和纯度分别为45%和99%左右。成果也为外消旋扁桃酸的手性拆分及苯乙酮酸的生物合成奠定了基础。

Abstract

Phenylglyoxylic acid (PGA) is key building block in the chemical synthesis

which could be used to synthesize a variety of important pharmaceutical intermediate

thus exploiting the green synthesis process of phenylacetone acid has significant economic value. The recombinant Escherichia coli whole cells containing the encoding gene of LhDMDH was used as catalyst in this study. The biotransformation of D-mandelic acid was researched under the condition of no coenzyme and cosubstrate addition. Subsequently

the transformed products were purified and identified. These results indicated that biosynthesis of phenylglyoxylic acid was realized under the condition of no coenzyme and cosubstrate addition. The yield and purity of PGA were 45% and 99%

respectively. In addition

this study established a solid foundation for chiral resolution of racemic mandelic acid and the biosynthesis of PGA.

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