Effect of counterions on protein adsorption to partial neutralization poly(ethylenimine)-grafted Sepharose FF

ZHAI Qiuhong; YU Linling; SUN Yan

doi:10.11949/j.issn.0438-1157.20170360

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Effect of counterions on protein adsorption to partial neutralization poly(ethylenimine)-grafted Sepharose FF

更新时间：2025-12-10

- Effect of counterions on protein adsorption to partial neutralization poly(ethylenimine)-grafted Sepharose FF
- CIESC Journal Issue 11, Pages: 4178-4185(2017)
- 作者机构：
  
  天津大学化工学院系统生物工程教育部重点实验室,天津,300072
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20170360
  CLC： Q033
- Online First：05 November 2017，
  
  Published：05 November 2017
- 稿件说明：
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ZHAI Qiuhong, YU Linling, SUN Yan. Effect of counterions on protein adsorption to partial neutralization poly(ethylenimine)-grafted Sepharose FF[J]. CIESC Journal, 2017, (11): 4178-4185.
DOI：

ZHAI Qiuhong, YU Linling, SUN Yan. Effect of counterions on protein adsorption to partial neutralization poly(ethylenimine)-grafted Sepharose FF[J]. CIESC Journal, 2017, (11): 4178-4185. DOI： 10.11949/j.issn.0438-1157.20170360.

摘要

前期研究表明部分电荷中和的聚乙烯亚胺接枝琼脂糖介质FF-PEI-R440具有较高静态吸附容量和快速传质速率。本文选取硫氰酸根离子、氯离子、磷酸氢根离子以及硫酸根离子这4种反离子，在离子强度为0.03、0.06 mol·L-1下，研究其对FF-PEI-R440吸附与洗脱行为的影响。结果表明：在两种离子强度下，饱和吸附容量的增加顺序均为SCN- < SO42- < HPO42- < Cl-；在低离子强度下，除SCN-外，反离子种类对传质速率和动态结合容量没有显著影响；在高离子强度下，传质速率的增加顺序为SCN- < SO42- ≈ Cl- < HPO42-，而动态结合容量的增加顺序与饱和吸附容量序列一致；两种离子强度下Cl-均保持最高的动态结合容量；反离子种类对蛋白质洗脱行为没有显著影响。上述结果说明反离子主要影响FF-PEI-R440的吸附性能，而不影响洗脱，且流动相中选择Cl-为反离子最利于FF-PEI-R440的实际柱色谱操作。

Abstract

The partially neutralized poly(ethylenimine)-grafted Sepharose FF

FF-PEI-R440

exhibited higher protein capacity and faster uptake rate than its starting resin FF-PEI-L740. In this work

the influence of counterions on protein adsorption onto and elution from the FF-PEI-R440 resin was investigated with sodium salts of SCN-

Cl-

HPO42- and SO42- at ionic strengths of 0.03 mol·L-1 and 0.06 mol·L-1. It was found that the static adsorption equilibrium of FF-PEI-R440 was significantly influenced by the counterions

and the adsorption capacity increased in the order of SCN- < SO42- < HPO42- < Cl- at the two ionic strengths. At the low ionic strength

the counterions had no significant effect on the uptake rate and dynamic binding capacity except for SCN-. At high ionic strength

the uptake rate increased in the order of SCN- < SO42-≈Cl- < HPO42-. Dynamic binding capacity increased in the order of SCN- < SO42- < HPO42- < Cl-

the same of the order of adsorption capacity. The dynamic binding capacity kept the highest values when Cl- existed at the two ionic strengths. Protein elution was little affected by counterions. The results indicated that the adsorption performance of FF-PEI-R440 was affected by counterions and the Cl- was the most favorable couterion for column operation.

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