Regulate properties of glucose oxidase biosensors through pore sizes of TiO<sub>2</sub> nanotube arrays

RAO Chao; DONG Yihui; ZHUANG Wei; WU Xinbing; HONG Qiliang; LIU Chang; LU Xiaohua

doi:10.11949/j.issn.0438-1157.20160181

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Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays

更新时间：2025-11-26

- Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays
- CIESC Journal Vol. 67, Issue 10, Pages: 4324-4333(2016)
- 作者机构：
  
  1. 南京工业大学材料化学工程国家重点实验室,江苏,南京,210009
  2. 南京工业大学国家生化工程技术研究中心,江苏,南京,211816
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20160181
  CLC： 647.32TB383
- Online First：05 October 2016，
  
  Published：05 October 2016
- 稿件说明：
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RAO Chao, DONG Yihui, ZHUANG Wei, et al. Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays[J]. CIESC Journal, 2016, 67(10): 4324-4333. DOI: 10.11949/j.issn.0438-1157.20160181.
DOI：

RAO Chao, DONG Yihui, ZHUANG Wei, et al. Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays[J]. CIESC Journal, 2016, 67(10): 4324-4333. DOI: 10.11949/j.issn.0438-1157.20160181. DOI：

摘要

采用电化学阳极氧化法制备出不同孔径（21、62、83、102 nm）的TiO2纳米管阵列（TNA），研究了孔径对固定化葡萄糖氧化酶（GOx）的传感器性能的影响。循环伏安测试结果表明固定在不同孔径大小的TNA上的GOx在葡萄糖溶液中均具有良好的酶活性。计时电流法和交流阻抗法测试发现，当孔径是83 nm时，灵敏度达到最大值27.2 μA·（mmol·L-1）-1·cm-2。调控TNA的孔径可改变固定化GOx的活性及溶液扩散阻抗，从而显著提高生物传感器性能。

Abstract

Highly ordered TiO2 nanotube arrays (TNA) with controlled pore sizes in a series of 21

83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption

and the GOx activities on TNA were investigated by cyclic voltammetry

chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L-1)-1·cm-2

which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

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Regulate properties of glucose oxidase biosensors through pore sizes of TiO2 nanotube arrays

DOI：10.11949/j.issn.0438-1157.20160181

摘要

Abstract

关键词

Keywords

references

Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays