Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere

TANG Zhimin; MA Xinbin

doi:10.11949/j.issn.0438-1157.20160176

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Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere

更新时间：2025-11-26

- Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere
- CIESC Journal Issue 6, Pages: 2410-2416(2016)
- 作者机构：
  
  天津大学化工学院,天津,300072
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20160176
  CLC： 927.2
- Online First：05 June 2016，
  
  Published：05 June 2016
- 稿件说明：
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TANG Zhimin, MA Xinbin. Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere[J]. CIESC Journal, 2016, (6): 2410-2416.
DOI：

TANG Zhimin, MA Xinbin. Preparation and adsorption properties of lysozyme molecularly imprinted polylactide microsphere[J]. CIESC Journal, 2016, (6): 2410-2416. DOI： 10.11949/j.issn.0438-1157.20160176.

摘要

以改性聚乳酸微球(MPLA)为载体

以γ-(2

3-环氧丙氧)丙基三甲氧基硅烷为功能单体

以四乙氧基硅烷为交联剂

采用溶胶凝胶方法制备了表面溶菌酶分子印迹改性聚乳酸微球(LZY-MIP-MPLA)

采用红外、扫描电镜和粒径测定等方法对LZY-MIP-MPLA进行了表征

优化了制备条件。详细研究了LZY-MIP-MPLA对溶菌酶的吸附性能

考察了pH和NaCl对吸附性能的影响。结果显示

LZY-MIP-MPLA对溶菌酶的吸附能力明显大于非印迹改性聚乳酸微球(NIP-MPLA)

达到吸附平衡的时间为200min左右。Scatchard方程的分析表明

印迹孔穴对模板分子的作用是不完全等价的

即存在两类不同的结合位点。LZY-MIP-MPLA对溶菌酶和牛血清白蛋白的分离因子为10.13

说明其对溶菌酶具有较好的选择吸附性能。

Abstract

Lysozyme molecularly imprinted modified polylactide microsphere (LZY-MIP-MPLA) was prepared by sol-gel using modified polylactide microsphere as carrier

3-epoxypropoxy propyltrimethoxysilicane as the functional monomer and tetraethyl orthosilicate as crosslinker. The prepared material was characterized by infrared spectroscopy and scanning electron microscopy. The preparation conditions were optimized. The adsorption properties of LZY-MIP-MPLA on lysozyme were studied in detail

and the influence of pH and NaCl on adsorption property was also investigated. The results showed that the adsorption capacity of LZY-MIP-MPLA on lysozyme was better than that of NIP-MPLA

and it needed 200 min to reach the equilibrium. By the analysis of Scatchard equation

the interactions of imprinted cavities and template molecule were not exactly equivalent and there existed two kinds of interaction places. The separation factor of LZY-MIP-MPLA on lysozyme and bovine albumin was 10.13

showing that LZY-MIP-MPLA displayed high recognition ability to the template molecule LZY.

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