Comparison of three silica resins in chromatographic property for mixed-mode chromatography

DONG Binbin; XIA Haifeng

doi:10.11949/j.issn.0438-1157.20160095

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Comparison of three silica resins in chromatographic property for mixed-mode chromatography

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- Comparison of three silica resins in chromatographic property for mixed-mode chromatography
- CIESC Journal Issue 7, Pages: 2893-2900(2016)
- 作者机构：
  
  1. 江南大学工业生物技术教育部重点实验室,江苏,无锡,214122
  2. 江南大学粮食发酵工艺与技术国家工程实验室,江苏,无锡,214122
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20160095
  CLC： P273
- Online First：05 July 2016，
  
  Published：05 July 2016
- 稿件说明：
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DONG Binbin, XIA Haifeng. Comparison of three silica resins in chromatographic property for mixed-mode chromatography[J]. CIESC Journal, 2016, (7): 2893-2900.
DOI：

DONG Binbin, XIA Haifeng. Comparison of three silica resins in chromatographic property for mixed-mode chromatography[J]. CIESC Journal, 2016, (7): 2893-2900. DOI： 10.11949/j.issn.0438-1157.20160095.

摘要

以γ-缩水甘油醚氧丙基三甲氧基硅烷为中间活化剂，将3-氨基吡啶、2-巯基苯并咪唑和2-巯基-1-甲基咪唑3种杂环配基键合到粒径为5 μm、孔径为30 nm的球形硅胶上，制备3种高效疏水电荷诱导色谱填料。利用溶菌酶、牛血清白蛋白和免疫球蛋白G作为模型蛋白，考察其在3种不同配基色谱柱上的保留行为和分离行为。比较了3种不同性质的模型蛋白在不同色谱柱上的色谱性能的异同。发现以2-巯基-1-甲基咪唑为配基的色谱柱具有良好的pH控制色谱行为和良好的分离性能，初步验证了高效疏水电荷诱导色谱的分离模式及潜在应用。

Abstract

Spherical silica gel with a diameter of 5 μm and pore size of 30 nm was activated by KH-560 and coupled with three kinds of ligands (3-aminopyridine

2-mercapto benzimidazole and 2-mercapto-1-methylimidazole) to prepare the high performance hydrophobic charge-induction chromatography (HPHCIC) column. The chromatographic properties of three columns were compared and evaluated with three model proteins (Lysozyme

BSA and IgG). The column with 2-mercapto-1-methylimidazole as ligand displayed a proper chromatography and separation behavior which controlled by pH. This work demonstrated that the novel synthetic materials can be utilized as the media of high performance hydrophobic charge induction chromatography.

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