Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H<sub>2</sub>/CO<sub>2</sub> in subcultures

LIU Chong; LI Jianzheng; ZHANG Yafei; ZHANG Yupeng; YOU Lijian; LIU Dongmei

doi:10.11949/j.issn.0438-1157.20151884

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Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H₂/CO₂ in subcultures

更新时间：2025-11-26

- Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H₂/CO₂ in subcultures
- CIESC Journal Issue 6, Pages: 2591-2597(2016)
- 作者机构：
  
  1. 哈尔滨工业大学市政环境工程学院,黑龙江,哈尔滨,150090
  2. 哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江,哈尔滨,150090
  3. 黑龙江大庆市大同区环保监测站,黑龙江,大庆,163515
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20151884
  CLC： 172
- Online First：05 June 2016，
  
  Published：05 June 2016
- 稿件说明：
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LIU Chong, LI Jianzheng, ZHANG Yafei, et al. Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H₂/CO₂ in subcultures[J]. CIESC Journal, 2016, (6): 2591-2597. DOI: 10.11949/j.issn.0438-1157.20151884.
DOI：

LIU Chong, LI Jianzheng, ZHANG Yafei, et al. Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H₂/CO₂ in subcultures[J]. CIESC Journal, 2016, (6): 2591-2597. DOI: 10.11949/j.issn.0438-1157.20151884. DOI：

摘要

为了解同型产乙酸菌异养代谢与自养代谢的相互作用与机制

并为快速获得具有较强自养代谢能力的菌体细胞提供培养方法

以H2/CO2和(或)葡萄糖为碳源

考察了Blautia coccoidesGA-1在连续传代培养中的代谢特征。结果表明

以H2/CO2作为唯一碳源进行连续传代培养时

菌株GA-1长势较弱

其子代的自养代谢能力也逐渐下降;在葡萄糖培养基中

菌株GA-1增殖旺盛

但高浓度的葡萄糖对其自养代谢能力有显著抑制作用

这种抑制作用可能是自养代谢和异养代谢对辅酶A和ATP的竞争、酸性环境造成的代谢抑制以及辅酶I的氧化还原平衡调节等综合作用的结果。以体积比为4:1的H2/CO2混合气为气相条件

用200mg·L-1葡萄糖培养基对菌株GA-1进行传代培养

不仅可获得稳定的子代培养物

而且可以将其利用H2/CO2产乙酸的能力维持在2.16g乙酸·(g干细胞)-1的水平。

Abstract

Though homoacetogens have been defined as autotrophic anaerobes using acetyl-CoA Wood-Ljungdahl carbon fixation pathway (WLP) for energy conservation

acetate production and biomass formation from CO2

these anaerobes can also utilize organic compounds heterotrophically to produce acetate and form biomass by Embden-Meyerhof-Parnas (EMP) glycolysis. A better understanding of the interrelationship between autotrophy and heterotrophy in homoacetogens will be helpful for acetate biosynthesis from exhaust rich in CO2. However

interaction of the two metabolisms has not been well understood though both of which have been extensively investigated respectively. Blautia coccoides GA-1

a novel homoacetogen strain obtained in a previous study

was introduced to the present research and the interrelationship between autotrophy and heterotrophy in the strain was investigated by sub-culturing with glucose and (or) H2/CO2 (vol

4:1) as carbon source (s). The results showed that the cell production of strain GA-1 in H2/CO2 cultures was too low to be sub-cultured after the 5th generation due to the energy produced was very limited in WLP. On the contrary

the strain could grow very well in glucose cultures and could be sub-cultured steadily. However

the autotrophic capability of the subcultures decreased by generations

even lost since the 7th generation in 1000 mg·L-1 glucose cultures. This may be resulted in: (1) the competition for Coenzyme A (CoA) between the autotrophic WLP and the heterotrophic EMP glycolysis

(2) ATP produced in the strain used up for biosynthesis rather for acetate synthesis from H2/CO2

(3) inhibition of lower pH less than 5.5 to the activity of hydrogenase

(4) negative effect of the lower pH on intracellular balance of NADH/NAD+. In mixotrophic cultures with H2/CO2 and 200 mg·L-1 glucose as co-carbon sources

strain GA-1 could not only be sub-cultured steadily but also keep autotrophic activity well in the generations with a specific acetate production of about 2.16 g·(g dry cell)-1 from H2/CO2.

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⁰

Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H2/CO2 in subcultures

DOI：10.11949/j.issn.0438-1157.20151884

摘要

Abstract

关键词

Keywords

references

Sub-culturing of novel Blautia coccoides GA-1 and acetate synthesis from H₂/CO₂ in subcultures