Immobilization of laccase on magnetic SiO<sub>2</sub> through dopamine self-polymerization for 4-CP removal

ZHANG Di; DENG Manfeng; ZHAO He; CAO Hongbin; ZHANG Songping

doi:10.11949/j.issn.0438-1157.20150871

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Immobilization of laccase on magnetic SiO₂ through dopamine self-polymerization for 4-CP removal

更新时间：2025-12-10

- Immobilization of laccase on magnetic SiO₂ through dopamine self-polymerization for 4-CP removal
- CIESC Journal Issue 9, Pages: 3705-3711(2015)
- 作者机构：
  
  1. 中国科学院过程工程研究所,北京,100190
  2. 天津化学化工协同创新中心,天津,300072
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20150871
  CLC： 703
- Online First：05 September 2015，
  
  Published：05 September 2015
- 稿件说明：
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ZHANG Di, DENG Manfeng, ZHAO He, et al. Immobilization of laccase on magnetic SiO₂ through dopamine self-polymerization for 4-CP removal[J]. CIESC Journal, 2015, (9): 3705-3711. DOI: 10.11949/j.issn.0438-1157.20150871.
DOI：

ZHANG Di, DENG Manfeng, ZHAO He, et al. Immobilization of laccase on magnetic SiO₂ through dopamine self-polymerization for 4-CP removal[J]. CIESC Journal, 2015, (9): 3705-3711. DOI: 10.11949/j.issn.0438-1157.20150871. DOI：

摘要

以磁性纳米颗粒为载体

通过多巴胺(dopamine

DA)聚合原位包埋制备了磁性SiO2固定化漆酶(Fe3O4@SiO2-PDA-Lac)。结果显示纳米颗粒尺寸均匀

并且保持较高的饱和磁性。通过最优条件制备出的固定化漆酶在50℃放置6 h后

活性保持在63%

而游离酶仅保留18%。将固定化酶用于催化降解4-氯酚(4-CP)

探究了溶液pH、漆酶浓度和ABTS[2

2-联氮-二(3-乙基-苯并噻唑-6-磺酸铵)]对4-CP去除率的影响。固定化漆酶在反应最适pH时

4-CP去除率为84.3%

而游离酶仅为65.7%。当漆酶浓度为1.2 U·ml-1时

反应8 h后

4-CP去除率可达95%

而游离酶的4-CP去除率仅82%。ABTS可促进固定化漆酶降解4-CP

当体系中加入50 μmol·L-1 的ABTS

反应10 min后

固定化酶对4-CP去除率可达99%。固定化漆酶在重复使用10次后

4-CP去除率仍可达67%。

Abstract

In recent years

laccase has been reported to be successfully immobilized on many different supports. Among them

potential applications of magnetic silica microspheres have been widely investigated for enzyme immobilization and the enzymatic stabilities can improve obviously. However

to the best of our knowledge

there is no report for the laccase immobilization on silica coated nanoparticles via one-step dopamine (DA) self-polymerization method

and nor any reports concerned with the degradation performance of paraphlorophenol (4-CP) by this immobilized laccase. In this work

the immobilized laccase on silica-coated magnetic nanoparticles were prepared through dopamine (DA) in situ entrapment

and then the immobilized laccase was applied for 4-chlorophenol (4-CP) removal. Briefly

the silica-coated magnetic nanoparticles were prepared through a sol-gel method

then the mixture of Fe3O4@SiO2 nanoparticles and DA was added to PBS (pH 6.0) containing laccase

the reaction was performed for 18 h at 25℃ with vigorous stirring

and was separated by an external magnetic field. After being washed 4 times

the product (Fe3O4@SiO2-PDA-Lac) was obtained. The scanning electron microscope images and size distribution analysis indicated that Fe3O4@SiO2 had a spherical and uniform size distribution with the average diameter of about 134 nm. The high saturation magnetization of Fe3O4@SiO2 made it easily to be separated from the reaction system. The results of X-ray diffraction (XRD) revealed that the prepared samples were Fe3O4 nanoparticles and Fe3O4@SiO2 nanoparticles. The preparing conditions of the Fe3O4@SiO2-PDA-Lac were optimized by orthogonal experiment. In the best level

the total activity recovery of the Fe3O4@SiO2-PDA-Lac can reach to 43.28%. In the degradation process

the effects of laccase concentration

solution pH and ABTS mediator on the removal efficiency of 4-CP were investigated. The results showed that when solution pH was 6 and laccase concentration was 1.2 U·ml-1

95% percentage of 4-CP was removed within 8 h enzymatic catalysis. When 50 μmol·L-1 of ABTS was added in the reaction system

the removal efficiency of 4-CP kept about 99% within 10 min enzymatic catalysis

which certified that the ABTS as a mediator could enhance the enzymatic reaction rate of the immobilized laccase. After degraded 10 times by the immobilized laccase

the removal efficiency of 4-CP still remained 67%

which exhibited an excellent reusability and operational stability.

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Immobilization of laccase on magnetic SiO2 through dopamine self-polymerization for 4-CP removal

DOI：10.11949/j.issn.0438-1157.20150871

摘要

Abstract

关键词

Keywords

references

Immobilization of laccase on magnetic SiO₂ through dopamine self-polymerization for 4-CP removal