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4-Butanetriol (BT) is an important organic synthetic intermediate. In this study

the metabolic network of Escherichia coli was reconstructed by heterogeneously expressing a keto acid decarboxylase (mdlC) from Pseudomonas putida ATCC12633 and a D-xylose dehydrogenase (xdh) from Caulobacter crescentus CB15

and knocking out xylA

yjhH and yagE which were the genes of xylose utilization pathway and intermediary metabolite pathway for D-1

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4-butanetriol synthesis. The recombinant strain could synthesize D-1

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4-butanetriol directly using D-xylose as precursor. Culture conditions such as temperature

medium volume

pH of fermentation broth were investigated at the titer of D-1

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4-butanetriol of 3.96 g·L-1 under suitable fermentation conditions. The relationship between glucose utilization and D-1

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4-butanetriol synthesis was discussed. After modifying the phosphoenolpyruvate: sugar phosphotransferase system (PTS) by knocking out ptsG the reconstructed E.coli could utilize glucose and xylose simultaneously

leading to a higher D-1

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4-butanetriol productivity.