Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system

WANG Weitao; JIANG Zhiguo; ZHANG Haide; PENG Jian; XU Yinghao; DONG Anhua; YANG Xuefang; JIANG Xinxin

doi:10.11949/j.issn.0438-1157.20140555

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Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system

更新时间：2025-12-10

- Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system
- CIESC Journal Issue 1, Pages: 179-185(2015)
- 作者机构：
  
  海南大学食品学院,海南,海口,570228
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20140555
  CLC： S255.1
- Online First：05 January 2015，
  
  Published：05 January 2015
- 稿件说明：
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WANG Weitao, JIANG Zhiguo, ZHANG Haide, et al. Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system[J]. CIESC Journal, 2015, (1): 179-185.
DOI：

WANG Weitao, JIANG Zhiguo, ZHANG Haide, et al. Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system[J]. CIESC Journal, 2015, (1): 179-185. DOI： 10.11949/j.issn.0438-1157.20140555.

摘要

采用离子液体双水相提取木瓜蛋白酶。首先考察不同浓度、pH、温度的离子液体对木瓜蛋白酶活性的影响

其次考察不同离子液体双水相体系、离子液体侧烷基链长度及浓度、酶添加量、pH、温度对木瓜蛋白酶分配行为的影响。结果表明:[C4mim]Cl和[C4mim]Br体系萃取木瓜蛋白酶的效果比[C4mim]BF4体系好;高温(≥60℃)对离子液体双水相体系萃取木瓜蛋白酶不利。离子液体双水相萃取木瓜蛋白酶的最佳工艺条件:0.25 g·ml-1的[C4mim]Cl

0.35 g·ml-1的K2HPO4

pH 8.0

酶添加量2.0 mg·ml-1

30℃。此条件下木瓜蛋白酶的酶活性回收率达到95.16%

纯化因子达到1.5。为今后进一步研究该体系的放大实验或规模化生产奠定了基础。

Abstract

Papain was extracted by ionic liquid aqueous two-phase system. Firstly

the influence of concentration

and temperature of ionic liquid on the activity of papain was investigated. Secondly

the effects of different ionic liquid aqueous two-phase systems

alkyl chain lengths and concentrations of the ionic liquid

dosage of papain

temperature on the partitioning behavior of papain were discussed. [C4mim]Cl and [C4mim]Br systems were better than [C4mim]BF4 system in extraction of papain

and it was disadvantageous to extract papain at a high temperature (60℃ or higher). Activity recovery of enzyme reached 95.16% and purification factor reached 1.5 under the optimum conditions: [C4mim]Cl 0.25 g·ml-1

K2HPO4 0.35 g·ml-1

pH 8.0

enzyme addition 2.0 mg·ml-1

30℃. The result laid the experimental basis for further scale-up research and commercial production.

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