Diethylamine/CuCl<sub>2</sub> pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency

PUYANG Xin; GUI Zheng; LIU Tingting; ZHANG Wenwen; YANG Wenjing; YANG Rongling; HE Aiyong; LUO Hongzhen

doi:10.11949/0438-1157.20260647

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Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency

更新时间：2026-07-08

- Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency
- CIESC Journal (2026)
- 作者机构：
  
  1.淮安大学生命科学与食品工程学院，江苏淮安 223003
  2.淮阴师范学院化学化工学院，江苏淮安 223300
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20260647
  CLC： TQ 352
- Received：11 May 2026，
  
  Revised：2026-07-07，
  
  Accepted：08 July 2026，
- 稿件说明：
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PUYANG Xin, GUI Zheng, LIU Tingting, et al. Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency[J/OL]. CIESC Journal, 2026. DOI: 10.11949/0438-1157.20260647.
DOI：

PUYANG Xin, GUI Zheng, LIU Tingting, et al. Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency[J/OL]. CIESC Journal, 2026. DOI: 10.11949/0438-1157.20260647. DOI：

摘要

木质纤维素是最丰富的可再生资源，可为燃料和生物基化学品生产提供发酵原料，但利用效率严重受限于其复杂的致密结构，通常需要预处理破解木质纤维素的抗降解屏障，提高纤维素酶对底物的可及性。基于此，利用二乙胺-水体系预处理玉米秸秆，考察不同条件对秸秆组分和酶解性能的影响。结果表明，使用40%二乙胺和60%水作为共溶剂，在110℃下处理2 h后的木质素脱除率达86.4%，经酶解后的葡萄糖得率为99.3%。在此基础上，加入20 mM路易斯酸氯化铜可有效降低预处理中的反应压力，固体残渣中的葡聚糖含量增至47.7%，酶解后葡萄糖产量达26.6 g/100 g玉米秸秆。表征结果表明，二乙胺/氯化铜预处理能够破坏秸秆表面结构，促进木质素和半纤维素脱除，提高纤维素可及性和酶解效率。综上，该预处理体系将为木质纤维素高效利用提供技术借鉴。

Abstract

Lignocellulosic biomass is the most abundant renewable resource. It is mainly composed of cellulose

hemicellulose

and lignin

and the polysaccharide components can be hydrolyzed into fermentable sugars. The association between polysaccharides and lignin in lignocellulose gives rise to the recalcitrant structure that impedes efficient degradation. Therefore

developing efficient pretreatment to deconstruct its structure is of great significance for achieving comprehensive biomass utilization. Herein

using corn stover as the raw material

pretreatment with diethylamine/water was conducted to investigate the effects of pretreatment conditions on compositional changes and enzymatic hydrolysis. The results showed that using 40% diethylamine and 60% water as co-solvent to pretreat corn stover at 110°C for 2 h achieved the lignin removal of 86.4% and glucose yield of 99.3% was obtained after 72 h enzymatic hydrolysis. Furthermore

the effects of Lewis acid addition were investigated. With the addition of 20 mM CuCl

the pressure during pretreatment was effectively reduced and the glucan content in the solid fraction reached 47.7%

and the glucose production after pretreatment and enzymatic hydrolysis increased to 26.6 g/100 g corn stover. Some characterization techniques were used to elucidate the mechanisms of diethylamine-based pretreatment that enhances t

he enzymatic hydrolysis efficiency of corn stover. Characterization results revealed that the pretreatment disrupted the dense surface structure of corn stover

promoted the removal of lignin and hemicellulose

and improved cellulose accessibility and enzymatic hydrolysis efficiency. In summary

this proposed pretreatment system provides a technical reference for the efficient utilization of lignocellulosic biomass.

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Diethylamine/CuCl2 pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency

DOI：10.11949/0438-1157.20260647

摘要

Abstract

关键词

Keywords

references

Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency