Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels

HE Yawei; ZHANG Songhong; HUANG Jie; LIU Liu; LI Guohua; YUN Junxian

doi:10.11949/0438-1157.20200623

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Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels

Separation engineering | 更新时间：2026-07-24

- Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels
- CIESC Journal Vol. 71, Issue 12, Pages: 5636-5643(2020)
- 作者机构：
  
  1.陕西能源职业技术学院，陕西咸阳 712000
  2.浙江工业大学化学工程学院，绿色化学合成技术国家重点实验室培育基地，浙江杭州 310032
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20200623
  CLC： TQ 028
- Received：21 May 2020，
  
  Revised：2020-07-07，
  
  Online First：17 December 2020，
  
  Published：05 December 2020
- 稿件说明：
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贺亚维, 张颂红, 黄杰, 刘流, 李国华, 贠军贤. 内嵌纳凝胶阴离子交换聚甲基丙烯酸羟乙酯复合晶胶分离苯乳酸研究[J]. 化工学报, 2020, 71(12): 5636-5643

HE Yawei, ZHANG Songhong, HUANG Jie, LIU Liu, LI Guohua, YUN Junxian. Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels[J]. CIESC Journal, 2020, 71(12): 5636-5643
贺亚维, 张颂红, 黄杰, 刘流, 李国华, 贠军贤. 内嵌纳凝胶阴离子交换聚甲基丙烯酸羟乙酯复合晶胶分离苯乳酸研究[J]. 化工学报, 2020, 71(12): 5636-5643 DOI： 10.11949/0438-1157.20200623.

HE Yawei, ZHANG Songhong, HUANG Jie, LIU Liu, LI Guohua, YUN Junxian. Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels[J]. CIESC Journal, 2020, 71(12): 5636-5643 DOI： 10.11949/0438-1157.20200623.

摘要

采用超声乳化聚合法制备了聚甲基丙烯酸丁酯纳凝胶，然后经结晶致孔法制备了内嵌纳凝胶的甲基丙烯酸羟乙酯纳晶胶，对其接枝功能单体

N，N，N

-三甲基乙烯基苯甲氯化铵，得到内嵌纳凝胶阴离子交换纳晶胶，用于从发酵液中分离苯乳酸。结果表明：所得纳晶胶具有良好的渗透性能和轴向分散性能，在流速为1 cm·min

-1

时，不同配比纳晶胶对牛血清白蛋白的吸附容量为3.9~5.4 mg·ml

-1

；将质量比为 8∶2（HEMA∶pBMA）的纳晶胶用于从转化液中分离苯乳酸，所得苯乳酸的纯度达89.24%，回收率93.74%，分离效果好，表明其在生物分离方面有良好的应用前景。

Abstract

Hydrophobic nanogels were synthesized by emulsion polymerization method under ultrasound using butyl methacrylate as the monomer. Then

anion exchange nano-cryogels embedded with the obtained nanogels were prepared by cryo-polymerization using poly(2-hydroxyethyl methacrylate) as the monomer

followed by a graft polymerization with the monomer (vinylbenzyl) trimethyl ammonium chloride. The anion exchange nano-cryogels were used to isolate phenyllactic acid from biotransformation broth. The results show that the obtained nanocrystalline gel has good permeability and axial dispersion performance. When the flow rate is 1 cm·min

-1

the adsorption capacity of different ratios of nanocrystalline gel for bovine serum protein is 3.9—5.4 mg·ml

-1

. When the nano-cryogel with mass ratio of 8∶2(HEMA∶pBMA) was used to isolate phenyllactic acid from transformation broth

phenyllactic acid with high purity of 89.24% and recovery of 93.74% was obtained

indicating that the nano-cryogels could have potential applications in bioseparation.

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references

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