Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants

Jiayuan HE; Zhuangfei JIANG; Rongrong MA; Lili YANG; Qingyao LI; Ling TAN; Zhitao CHEN; Qihui ZHANG

doi:10.11949/0438-1157.20200346

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Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants

Separation engineering | 更新时间：2025-11-25

- Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants
- CIESC Journal Vol. 71, Issue 10, Pages: 4711-4719(2020)
- 作者机构：
  
  1.重庆大学化学化工学院，重庆 400044
  2.重庆大学药学院，重庆 400044
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20200346
  CLC： R 917
- Received：01 April 2020，
  
  Revised：2020-05-22，
  
  Published：05 October 2020
- 稿件说明：
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何家垣, 蒋壮飞, 马蓉蓉, 杨莉莉, 李清瑶, 谭玲, 陈志涛, 张起辉. 磁性温敏分子印迹材料用于药用植物中富集单一成分——芒柄花黄素的分离与富集[J]. 化工学报, 2020, 71(10): 4711-4719

HE Jiayuan, JIANG Zhuangfei, MA Rongrong, YANG Lili, LI Qingyao, TAN Ling, CHEN Zhitao, ZHANG Qihui. Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants[J]. CIESC Journal, 2020, 71(10): 4711-4719
何家垣, 蒋壮飞, 马蓉蓉, 杨莉莉, 李清瑶, 谭玲, 陈志涛, 张起辉. 磁性温敏分子印迹材料用于药用植物中富集单一成分——芒柄花黄素的分离与富集[J]. 化工学报, 2020, 71(10): 4711-4719 DOI： 10.11949/0438-1157.20200346.

HE Jiayuan, JIANG Zhuangfei, MA Rongrong, YANG Lili, LI Qingyao, TAN Ling, CHEN Zhitao, ZHANG Qihui. Magnetic temperature-sensitive molecularly imprinted materials for separation and enrichment of single component of formononetin in medicinal plants[J]. CIESC Journal, 2020, 71(10): 4711-4719 DOI： 10.11949/0438-1157.20200346.

摘要

以四氧化三铁（Fe

）纳米粒子为支撑材料，模板选用芒柄花黄素，温敏功能单体和辅助功能单体分别选用

-异丙基丙烯酰胺（NIPAM）和甲基丙烯酸（MAA），制备出磁性和温度双响应型分子印迹材料（MTMIPs），并结合高效液相色谱法（HPLC）将其用于红车轴草中芒柄花黄素的定向萃取研究。利用扫描电镜（SEM）、透射电镜（TEM）、红外光谱（FT-IR）、热重分析（TGA）、磁性分析（VSM）等手段对MTMIPs进行表征，随后对其温敏及吸附性能进行考察。结果表明，MTMIPs为核壳型结构，热稳定性好，具有良好的吸附性能（16.43 mg/g）和温敏特性，对芒柄花黄素的吸附动力学符合准二级动力学模型，吸附过程符合Langmuir单分子层吸附。HPLC检测结果显示MTMIPs可用于从复杂样品中分离富集芒柄花黄素。

Abstract

The magnetic and temperature double response molecularly imprinted polymers for formononetin-specific adsorption (MTMIPs) were successfully prepared by using Fe

as supporting matrix

formononetin as template

-isopropyl acrylamide as the thermo-sensitive type functional monomer and methacrylic acid as auxiliary functional monomer. SEM

TEM

FT-IR

TGA and magnetic analysis were used to characterize the structure of MTMIPs

and then their adsorption properties and reproducibility were investigated. The results showed that MTMIPs was a core-shell structure with good thermal stability

good adsorption performance (16.43 mg/g) and fast adsorption performance. The adsorption kinetics of formononetin was consistent with the quasi-second-order kinetics model

and the adsorption process was consistent with the Langmuir monolayer adsorption with good reproducibility. HPLC test results show that MTMIPs can be used to separate and enrich formononetin from complex samples.

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references

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