Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor

DENG Qiujin; SU Chengyuan; LU Xinya; GUAN Xin; QIN Ronghua; DENG Yulian; GAO Shu; HUANG Zun

doi:10.11949/0438-1157.20200273

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Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor

Energy and environmental engineering | 更新时间：2026-07-24

- Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor
- CIESC Journal Vol. 71, Issue 12, Pages: 5802-5812(2020)
- 作者机构：
  
  1.珍稀濒危动植物生态与环境保护教育部重点实验室，广西桂林 541004
  2.广西师范大学环境与资源学院，广西桂林 541004
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20200273
  CLC： X 703.1
- Received：16 March 2020，
  
  Revised：2020-05-05，
  
  Online First：17 December 2020，
  
  Published：05 December 2020
- 稿件说明：
移动端阅览
邓秋金, 宿程远, 陆欣雅, 关鑫, 覃容华, 邓钰莲, 高澍, 黄尊. 氮浓度对新型生物电化学-颗粒污泥反应器运行的影响[J]. 化工学报, 2020, 71(12): 5802-5812

DENG Qiujin, SU Chengyuan, LU Xinya, GUAN Xin, QIN Ronghua, DENG Yulian, GAO Shu, HUANG Zun. Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor[J]. CIESC Journal, 2020, 71(12): 5802-5812
邓秋金, 宿程远, 陆欣雅, 关鑫, 覃容华, 邓钰莲, 高澍, 黄尊. 氮浓度对新型生物电化学-颗粒污泥反应器运行的影响[J]. 化工学报, 2020, 71(12): 5802-5812 DOI： 10.11949/0438-1157.20200273.

DENG Qiujin, SU Chengyuan, LU Xinya, GUAN Xin, QIN Ronghua, DENG Yulian, GAO Shu, HUANG Zun. Influence of nitrogen concentration on operation of a novel bio-electrochemical-granular sludge reactor[J]. CIESC Journal, 2020, 71(12): 5802-5812 DOI： 10.11949/0438-1157.20200273.

摘要

探讨了新型生物电化学-颗粒污泥反应器在不同进水氮浓度下的脱氮效能与产电性能，并从颗粒污泥的关键酶活性、胞外聚合物组分以及微生物群落分布等角度系统研究了其影响机制。结果表明，COD、NO

-N、NO

-N和溶解性甲烷在第Ⅰ、Ⅱ、Ⅲ、Ⅳ阶段（进水NO

-N和NO

-N浓度分别为60 mg·L

-1

和20 mg·L

-1

、100 mg·L

-1

和40 mg·L

-1

、140 mg·L

-1

和60 mg·L

-1

、180 mg·L

-1

和80 mg·L

-1

）均得以有效去除，其中COD去除率在第Ⅳ阶段效果最佳，去除率达96%以上，NO

-N出水浓度在第Ⅱ阶段更为稳定，其去除率达99%以上，NO

-N去除率在各阶段均达99%以上；该反应器最大的功率密度与输出电压值为第Ⅳ阶段的4号格室，分别为471.2 mV·m

-3

和608.1 mV。污泥疏松型胞外聚合物（LB-EPS）中多糖与蛋白含量最高为第Ⅱ阶段的5号格室，分别为13.7 mg·g

-1

和14.7 mg·g

-1

；1号格室污泥中辅酶F

420

活性最低，进水氮浓度的增大提高了污泥中蛋白酶活性。由第Ⅰ阶段至第Ⅳ阶段，该反应器中变形菌门（Protebaoteria）相对丰度减少，而绿弯菌门（Chloroflexi）、厚壁菌门（Firmicutes）和浮霉菌门（Planctomycetes）相对丰度增加；具有脱氮作用的陶厄氏菌属（

Thauera

）在1号格室减少了8.64%，但该反应器脱氮效果未受到影响；甲烷丝状菌属（

Methanothrix

）在4号格室相对丰度增至12.3%，表明产甲烷菌可在该反应器中与其他菌群联营共存。

Abstract

The performance of nitrogen removal and electricity generation of a novel bio-electrochemical-granular sludge reactor at different influent nitrogen concentration was investigated. The impact mechanism of granular sludge

key enzyme activity

extracellular polymer composition and microbial community

distribution were systematically studied. The results showed that COD

-N

-N and dissolved methane were efficiently removed in stages Ⅰ

Ⅱ

Ⅲ and Ⅳ (Influent NO

-N and NO

-N concentrations were 60 and 20 mg·L

-1

100 and 40 mg·L

-1

140 and 60 mg·L

-1

180 and 80 mg·L

-1

respectively). The removal efficiency of COD was the highest in stage Ⅳ

and it was above 96%. The effluent concentration of NO

-N was the most stable at stage Ⅱ

and the removal efficiency was over 99%. The NO

-N removal efficiency was above 99% in each stage. In stage Ⅳ

the maximum power density and output voltage was 471.2 mV·m

-3

and 608.1 mV at the fourth compartment

respectively. The polysaccharide and protein content of LB-EPS was the highest in stage Ⅱ of the fifth compartment

13.7 mg·g

-1

and 14.7 mg·g

-1

respectively. Coenzyme F

420

activity was the lowest in the first compartment. The protease activity of the sludge was increased due to the increase of influent nitrogen concentration. From stage Ⅰ to stage Ⅳ

the relative abundance of Protebaoteria was decreased

while the relative abundance of Chloroflexi

Firmicutes and Planctomycetes were increased. Although

Thauera

with denitrification effect was decreased by 8.64% in the first compartment

the nitrogen removal was still well in the reactor. The relative abundance of

Methanothrix

was increased to 12.3% in the fourth compartment

indicating that

Methanothrix

could co-exist with other bacteria in the reactor.

关键词

Keywords

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