Variation of N<sub>2</sub>O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

GONG Youkui; ZHAO Qiang; PENG Yongzhen

doi:10.11949/0438-1157.20190753

您当前的位置：

首页 >

文章列表页 >

Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

Energy and environmental engineering | 更新时间：2026-07-24

- Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio
- CIESC Journal Vol. 70, Issue 12, Pages: 4847-4855(2019)
- 作者机构：
  
  1. 烟台职业学院建筑工程系，山东烟台 264670
  2. 北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20190753
  CLC： X 703.1
- Received：02 July 2019，
  
  Revised：2019-08-28，
  
  Online First：09 June 2020，
  
  Published：05 December 2019
- 稿件说明：
移动端阅览
巩有奎, 赵强, 彭永臻. 不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化[J]. 化工学报, 2019, 70(12): 4847-4855 DOI: 10.11949/0438-1157.20190753.

GONG Youkui, ZHAO Qiang, PENG Yongzhen. Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio[J]. CIESC Journal, 2019, 70(12): 4847-4855 DOI: 10.11949/0438-1157.20190753.
巩有奎, 赵强, 彭永臻. 不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化[J]. 化工学报, 2019, 70(12): 4847-4855 DOI: 10.11949/0438-1157.20190753. DOI：

GONG Youkui, ZHAO Qiang, PENG Yongzhen. Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio[J]. CIESC Journal, 2019, 70(12): 4847-4855 DOI: 10.11949/0438-1157.20190753. DOI：

摘要

在(20±2.0)℃条件下，利用序批式生物膜反应器（sequencing batch biofilm reactor，SBBR），考察不同碳氮比（C/N=3.0、5.0、8.0和10.0）下同步脱氮（simultaneous nitrification and denitrification，SND）过程N

O释放及胞外聚合物（extracellular polymeric substance，EPS）变化。C/N由3.0增至10.0，异养菌大量增殖，曝气阶段DO降低，系统硝化性能受到抑制，SBBR系统出水NH

由0.5 mg/L以下增至（7.85±1.42）mg/L，N

O产量由（2.68±0.17）mg/L降至（1.02±0.12）mg/L。C/N=8.0，TN去除率最大为80.4%±3.5%。反应初期，微生物体内聚β-羟基烷酸酯（PHA）增加，可为后续反硝化过程提供电子供体。AOB好氧反硝化和低氧条件下异养菌反硝化过程均可导致N

O产生。C/N降低，SBBR内部缺氧区域减少，N

O还原过程减弱，释

放量增加；C/N增加，N

O扩散进入生物膜内缺氧区域，促进其减量。C/N由3.0增至10.0，微生物EPS分泌由（57.6±5.6）mg / g VSS降至（32.7±3.2）mg / g VSS，其中，TB-EPS含量占65.8%~68.8%。低C/N下，紧密型EPS（TB-EPS）中多糖（PS）含量增加，生物膜更加密实，N

O扩散进入缺氧区阻力增加，释放量增加。

Abstract

At normal temperature of (20±2.0)℃

the characteristics of N

O eimssion and extracellular polymeric substance(EPS) production were studied during simultaneous nitrification and denitrification(SND) process under different C/N ratio (C/N=3.0

5.0

8.0 and 10.0). When C/N increased from 3.0 to 10.0

the heterotrophic bacteria increased rapidly

DO decreased and the nitrification process was inhibited. The effluent NH

increased from below 0.5 mg/L to (7.85±1.42) mg/L

and the N

O emission decreased from (2.68±0.17) mg/L to (1.02±0.12) mg/L. When C/N was 8.0

the maximum total notrogen removal efficiency was 80.4%±3.5%. The accumulation of PHA(poly-β-hydroxyalkanoate) increased firstly and then decreased. Both nitrifier denitrification and heterotrophic denitrification resulted in N

O production. The anoxic area located in the biofilm decreased with the decreasing of C/N ratio

which weakened the denitrification process. With the increase of C/N ratio

O diffused into the anoxic region of biofilm can be effectively reduced. The microbial EPS secretion decreased from (57.6±5.6) mg / g VSS to (32.7±3.2) mg / g VSS. The TB-EPS content accounts for 65.8%—68.8% of the total EPS. Under low C/N

the content of polysaccharide (PS) in compact EPS (TB-EPS) increased

the biofilm was more compact

the resistance of N

O diffusion into the anoxic zone increased

and the release increased.

关键词

Keywords

references

Wang J , Rong H , Zhang C . Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor [J ] . Journal of Bioscience and Bioengineering , 2018 , 125 ( 5 ): 532 - 542 .

Zhao J , Feng L , Yang G , et al . Development of simultaneous nitrification-denitrification (SND) in biofilm reactors with partially coupled a novel biodegradable carrier for nitrogen-rich water purification [J ] . Bioresource Technology , 2017 , 243 : 800 - 809 .

Sabba F , Terada A , Wells G , et al . Nitrous oxide emissions from biofilm processes for wastewater treatment [J ] . Applied Microbiology and Biotechnology , 2018 , 102 ( 22 ): 9815 - 9829 .

Mahne I , Princic A , Megusar F . Nitrification/denitrification in nitrogen high-strength liquid wastes [J ] . Water Research , 1996 , 30 ( 9 ): 2107 - 2111 .

Her J J , Huang J S . Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough [J ] . Bioresource Technology , 1995 , 54 ( 1 ): 45 - 51 .

Wang X , Chen Z , Shen J , et al . Impact of carbon to nitrogen ratio on the performance of aerobic granular reactor and microbial population dynamics during aerobic sludge granulation [J ] . Bioresource Technology , 2019 , 271 : 258 - 265 .

Chao C , Zhao Y , Keskar J , et al . Simultaneous removal of COD , nitrogen and phosphorus and the tridimensional microbial response in a sequencing batch biofilm reactor: with varying C/N/P ratios [J/OL ] .Biochemical Engineering Journal, https: // doi . org/ 10 .1016/j.bej. 2019 . 04 .017.

Ye F X , Ye Y F , Li Y . Effect of C/N ratio on extracellular polymeric substances (EPS) and physicochemical properties of activated sludge flocs [J ] . Journal of Hazardous Materials , 2011 , 188 ( 1 / 2 / 3 ): 37 - 43 .

Wang Z , Gao M , Xin Y , et al . Effect of C/N ratio on extracellular polymeric substances of activated sludge from an anoxic-aerobic sequencing batch reactor treating saline wastewater [J ] . Environmental Technology , 2014 , 35 ( 24 ): 2821 - 2828 .

Ravishankara A R , Daniel J S , Portmann R W . Nitrous oxide(N 2 O): the dominant ozone-depleting substance emitted in the 21st century [J ] . Science , 2009 , 326 ( 5949 ): 123 - 125 .

Ye L , Ni B J , Law Y Y , et al . A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators [J ] . Water Research , 2014 , 48 ( 1 ): 257 - 268 .

Stefano M , Giaime T , Giovannimatteo E . Evaluation of nitrous oxide gaseous emissions from a partial nitritation reactor operating under different conditions [J ] . Desalination and Water Treatment , 2018 , 127 : 158 - 164 .

Kong Q , Wang Z , Niu P , et al . Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process [J ] . Bioresource Technology , 2016 , 210 : 94 - 100 .

Jia W , Liang S , Zhang J , et al . Nitrous oxide emission in low-oxygen simultaneous nitrification and denitrification process: sources and mechanisms [J ] . Bioresource Technology , 2013 , 136 : 444 - 451 .

APHA(American Public Health Association) . Standard methods for the examination of water and wastewater [S ] . Baltimore : Port City Press , 1998 .

Oehmen A , Keller-Lehmann B , Zeng R J , et al . Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J ] . Journal of Chromatography A , 2005 , 1070 ( 1 / 2 ): 131 - 136 .

Yang Q , Liu X H , Peng C Y , et al . N 2 O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method [J ] . Environmental Science & Technology , 2009 , 43 ( 24 ): 9400 - 9406 .

张建华 , 彭永臻 , 张淼 , 等 . 同步硝化反硝化SBBR处理低C/N比生活污水的启动与稳定运行 [J ] . 化工学报 , 2016 , 67 ( 11 ): 4817 - 4824 .

Zhang J H , Peng Y Z , Zhang M , et al . Start-up and steady operation of simultaneous nitrification and denitrification in SBBR treating low C/N ratio domestic wastewater [J ] . CIESC Journal , 2016 , 67 ( 11 ): 4817 - 4824 .

巩有奎 , 任丽芳 , 彭永臻 . 不同DO下SBBR亚硝酸型同步脱氮及N 2 O释放特性 [J ] . 化工学报 , 2019 , 70 ( 4 ): 1550 - 1558 .

Gong Y K , Ren L F , Peng Y Z . Characteristics of simultaneous nitrification and denitrification via nitrite and N 2 O emission in SBBR under different DO concentrations [J ] . CIESC Journal , 2019 , 70 ( 4 ): 1550 - 1558 .

王荣昌 , 肖帆 , 赵建夫 . 生物膜厚度对膜曝气生物膜反应器硝化性能的影响 [J ] . 高校化学工程学报 , 2015 , 29 ( 1 ): 151 - 158 .

Wang R C , Xiao F , Zhao J F . Effects of biofilm thickness on nitrification performance of membrane-aerated biofilm reactors [J ] . Journal of Chemical Engineering of Chinese Universities , 2015 , 29 ( 1 ): 151 - 158 .

Bradford M M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J ] . Analytical Biochemistry , 1976 , 72 ( 1 ): 248 - 254 .

Dubois M , Gilles K A , Hamilton J K , et al . Colorimetric method for determination of sugars and related substances [J ] . Analytical Chemistry , 1956 , 28 ( 3 ): 350 - 356 .

戴娴 , 王晓霞 , 彭永臻 , 等 . 进水C /N 对富集聚磷菌的SNDPR系统脱氮除磷的影响 [J ] . 中国环境科学 , 2015 , 35 ( 9 ): 2636 - 2643 .

Dai X , Wang X X , Peng Y Z , et al . Effect of influent C/N ratio on simultaneous nitrification-denitrification and phosphorus removal (SNDPR) enriched with phosphorus accumulating organisms (PAOs) [J ] . China Environmental Science , 2015 , 35 ( 9 ): 2636 - 2643 .

Nogueira R , Melo L F , Purkhold U , et al . Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon [J ] . Water Research , 2002 , 36 ( 2 ): 469 - 481 .

赵青 , 卞伟 , 李军 , 等 . DO/NH 4 + -N实现短程硝化过程中生物膜特性 [J ] . 环境科学 , 2018 , 39 ( 3 ): 1278 - 1285 .

Zhao Q , Bian W , Li J , et al . Characteristics of biofilm during the transition process of complete nitrification and partial nitrification [J ] . Environmental Science , 2018 , 39 ( 3 ): 1278 - 1285 .

Yang S , Yang F L , Fu Z M , et al . Simultaneous nitrogen and phosphorus removal by a novel sequencing batch moving bed membrane bioreactor for wastewater treatment [J ] . Journal of Hazardous Materials , 2010 , 175 ( 1 / 2 / 3 ): 551 - 557 .

Peng L , Ni B J , Erler D , et al . The effect of dissolved oxygen on N 2 O production by ammonia-oxidizing bacteria in an enriched nitrifying sludge [J ] . Water Research , 2014 , 66 ( 1 ): 12 - 21 .

Gong Y , Peng Y , Yang Q , et al . Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate [J ] . Journal of Hazardous Materials , 2012 , 227 / 228 : 453 - 460 .

Zeng R J , Romain L , Yuan Z , et al . Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor [J ] . Biotechnology & Bioengineering , 2003 , 84 ( 2 ): 170 - 178 .

曾薇 , 张悦 , 李磊 . 限氧曝气实现亚硝酸型同步硝化反硝化的研究 [J ] . 北京工业大学学报 , 2010 , 36 ( 9 ): 1263 - 1270 .

Zeng W , Zhang Y , Li L . Simultaneous nitrification denitrification via nitrite under limited aeration [J ] . Journal of Beijing University of Technology , 2010 , 36 ( 9 ): 1263 - 1270 .

Letey J , Valoras N , Focht D D , et al . Nitrous oxide production and reduction during denitrification as affected by redox potential [J ] . Soil Science Society of America Journal , 1981 , 45 ( 4 ): 727 - 730 .

刘越 , 李鹏章 , 彭永臻 , 等 . 短程硝化过程中硝化速率与N 2 O产生速率的关系 [J ] . 化工学报 , 2015 , 66 ( 11 ): 4652 - 4660 .

Liu Y , Li P Z , Peng Y Z , et al . Relationship between N 2 O production rate and ammonia oxidation rate during nitritation process [J ] . CIESC Journal , 2015 , 66 ( 11 ): 4652 - 4660 .

Chen X , Yuan Z , Ni B J . Nitrite accumulation inside sludge flocs significantly influencing nitrous oxide production by ammonium-oxidizing bacteria [J ] . Water Research , 2018 , 143 ( 15 ): 99 - 108 .

Rusanowska P , Cydzik-Kwiatkowska A , Świątczak P , et al . Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads [J ] . Bioresource Technology , 2019 , 272 : 188 - 193 .

Kang J A , Yuan Q . Long-term stability and nutrient removal efficiency of aerobic granules at low organics loads [J ] . Bioresource Technology , 2017 , 234 : 336 - 342 .

孙洪伟 , 陈翠忠 , 高宇学 , 等 . 碳氮比对活性污泥胞外聚合物的长期影响 [J ] . 中国环境科学 , 2018 , 38 ( 3 ): 950 - 958 .

Sun H W , Chen C Z , Gao Y X , et al . Effect of C/N ratio on extracellular polymeric substance (EPS) in the sequencing batch reactor (SBR) [J ] . China Environmental Science , 2018 , 38 ( 3 ): 950 - 958 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of aeration intensity on simultaneous biological nitrogen removal and N2O release from SBBR

Study on the synergistic nitrogen removal mechanisms between biofilms and activated sludge and the low-carbon operational characteristics of MABR

The characteristics of SNDPR and N2O emission in anaerobic-oxygen limited SBR treating low C/N domestic sewage

Effect of operation mode on characteristics and N2O emissions during simultaneous nitrification and denitrification via nitrite in SBBR

Characteristics of simultaneous nitrification and denitrification via nitrite and N2O emission in SBBR under different DO concentrations

Related Author

Yongzhen PENG

Youkui GONG

QIAN Feiyue

LIU Feng

YUAN Longhao

HU Yang

LU Meijie

HONG Jinbo

Related Institution

School of Environmental Science and Engineering, Suzhou University of Science and Technology

Suzhou Kete Environmental Protection Co., Ltd.

School of Environmental and Material Engineering, Yantai University

School of Architecture and Civil Engineering, Beijing University of Technology

⁰

Variation of N2O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

DOI：10.11949/0438-1157.20190753

摘要

Abstract

关键词

Keywords

references

Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio