Domestication and identification of cold-resistant bacteria in SBBR reactor

WU Han; CHEN Ying; LIU Min; WANG Shuying; ZHANG Wei

doi:10.11949/0438-1157.20190731

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Domestication and identification of cold-resistant bacteria in SBBR reactor

Energy and environmental engineering | 更新时间：2026-07-24

- Domestication and identification of cold-resistant bacteria in SBBR reactor
- CIESC Journal Vol. 71, Issue 2, Pages: 766-776(2020)
- 作者机构：
  
  1. 四川大学建筑与环境学院，四川成都 610065
  2. 北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124
  3. 四川大学高分子研究所高分子材料工程国家重点实验室，四川成都 610065
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20190731
  CLC： X 703
- Received：01 July 2019，
  
  Revised：2019-07-31，
  
  Online First：14 August 2020，
  
  Published：05 February 2020
- 稿件说明：
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吴涵, 陈滢, 刘敏, 王淑莹, 张伟. SBBR反应器中耐冷微生物的驯化与识别[J]. 化工学报, 2020, 71(2): 766-776

WU Han, CHEN Ying, LIU Min, WANG Shuying, ZHANG Wei. Domestication and identification of cold-resistant bacteria in SBBR reactor[J]. CIESC Journal, 2020, 71(2): 766-776
吴涵, 陈滢, 刘敏, 王淑莹, 张伟. SBBR反应器中耐冷微生物的驯化与识别[J]. 化工学报, 2020, 71(2): 766-776 DOI： 10.11949/0438-1157.20190731.

WU Han, CHEN Ying, LIU Min, WANG Shuying, ZHANG Wei. Domestication and identification of cold-resistant bacteria in SBBR reactor[J]. CIESC Journal, 2020, 71(2): 766-776 DOI： 10.11949/0438-1157.20190731.

摘要

以模拟生活污水为处理对象，三种不同填料为生物膜载体，运行 A、B、C三个SBBR，通过逐步降温（25、20、15、10、6和5℃）培养驯化处理低温污水的生物膜。运行251周期，三个反应器COD出水浓度均低于50 mg/L；B反应器对氨氮去除效果优于A和C。特别在低温5℃时，A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示，5℃时，三个反应器中均以降解有机物的微生物为主；只有B反应器中驯化富集出了嗜冷硝化菌（

Candidatus Nitrotoga

）；而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器；不利于脱氮的类固氮菌（

Elstera

）在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性，表明B反应器处理低温污水有显著优势，其所用填料可结合低温菌进一步开发利用。

Abstract

Taking simulated domestic sewage as the treatment object

three different fillers are biofilm carriers

and three SBBRs of A

B and C are operated

and the biofilm of low temperature sewage is domesticated and treated by gradual cooling (25

6 and 5℃). During the 251 operating cycles

the COD effluent concentration of the three reactors was less than 50 mg/L. The ammonium removal efficiency of the B reactor was better than A and C. At 5℃

the ammonium concentration of effluent of A

B and C were 14.1

3.79 and 14.1 mg/L

respectively. The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly

those can degrade organic matter at 5℃. Psychrophilic nitrifying bacteria (

Candidatus Nitrotoga

) were found only in B reactor. The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors. The nitrogen-fixing bacteria (

Elstera

)

which adverse to remove ammonium，has the minimal distribution in the B reactor. These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism

which shows that B reactor has significant advantages in treating low temperature wastewater.

关键词

Keywords

references

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