Cell factory construction accelerated by genome synthesis and rearrangement

XIE Zexiong; CHEN Xiangrong; XIAO Wenhai; LI Bingzhi; YUAN Yingjin

doi:10.11949/0438-1157.20190702

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Cell factory construction accelerated by genome synthesis and rearrangement

Reviews and monographs | 更新时间：2026-07-24

- Cell factory construction accelerated by genome synthesis and rearrangement
- CIESC Journal Vol. 70, Issue 10, Pages: 3712-3721(2019)
- 作者机构：
  
  1.教育部合成生物学前沿科学中心，天津 300072
  2.天津大学化工学院系统生物工程教育部重点实验室，天津 300072
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20190702
  CLC： TQ 033
- Received：21 June 2019，
  
  Revised：2019-08-31，
  
  Online First：24 July 2026，
  
  Published：05 October 2019
- 稿件说明：
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谢泽雄, 陈祥荣, 肖文海, 李炳志, 元英进. 基因组再造与重排构建细胞工厂[J]. 化工学报, 2019, 70(10): 3712-3721

XIE Zexiong, CHEN Xiangrong, XIAO Wenhai, LI Bingzhi, YUAN Yingjin. Cell factory construction accelerated by genome synthesis and rearrangement[J]. CIESC Journal, 2019, 70(10): 3712-3721
谢泽雄, 陈祥荣, 肖文海, 李炳志, 元英进. 基因组再造与重排构建细胞工厂[J]. 化工学报, 2019, 70(10): 3712-3721 DOI： 10.11949/0438-1157.20190702.

XIE Zexiong, CHEN Xiangrong, XIAO Wenhai, LI Bingzhi, YUAN Yingjin. Cell factory construction accelerated by genome synthesis and rearrangement[J]. CIESC Journal, 2019, 70(10): 3712-3721 DOI： 10.11949/0438-1157.20190702.

摘要

细胞工厂能利用微生物细胞制备人类所需能源、药物和化学品。底盘细胞和外源代谢路径的适配是构建高效细胞工厂的核心难题。基因组再造指利用化学合成的核苷酸分子“自下而上”构建生物基因组，基因组诱导重排指通过在全基因组尺度进行DNA序列与结构的人为调控。基因组再造和诱导重排实现了对生命体的创造，增强了模式底盘细胞的遗传稳定性和操作柔性。基因组的适度精简和密码子简化改善细胞对底物、能量的利用效率，提高细胞生理性能的预测性和可控性。基因组重排可诱导染色体发生随机删除、复制、移位和倒置等结构变异，可产生大量性状优良的模式底盘细胞，进而加速代谢路径优化，提高路径和底盘细胞的适配性，为人工细胞工厂快速构建和优化提供了新策略。

Abstract

Cell factories can use microbial cells to produce the energy

drugs

and chemicals that humans need. The adaptation of chassis cells to exogenous metabolic pathways is a core challenge in building efficient cell factories. Genome synthesis refers to the bottom-up construction of a genome from chemically synthesized nucleotides. Genomic induced rearrangement refers to the customized regulation of DNA sequences and structures in genome scale. Genome synthesis and inducible rearrangement have enabled the creation of organisms

enhanced the flexibility of model chassis. Proper genome simplification and codon simplification improve the utilization efficiency of substrates and energy

and improve the predictability and controllability of cell physiological performance. Genomic rearrangement can generate structural variations such as random deletion

replication

translocation and inversion. The rearrangements of synthetic genome accelerate the rapid evolution of chassis and the optimization of metabolic pathways

and improve the adaptability between chassis and pathway. The genome synthesis and inducible rearrangement provide a new strategy for the construction and optimization of cell factories.

关键词

Keywords

references

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